In recent years, the advent of intestinal organoid culture systems has revolutionized in vitro
studies of the small intestine epithelium. Intestinal organoids are derived from self …

Barrier tissue dysfunction is a fundamental feature of chronic human inflammatory diseases
1. Specialized subsets of epithelial cells—including secretory and ciliated cells …

Cellular diversity that shapes tissue architecture and function is governed by multiple niche
signals. Nonetheless, maintaining cellular diversity in human intestinal organoids has been …

Organoids are complex three-dimensional in vitro organ-like model systems. Human
organoids, which are derived from human pluripotent stem cells or primary human donor …

There is pressing urgency to understand the pathogenesis of the severe acute respiratory
syndrome coronavirus clade 2 (SARS-CoV-2), which causes the disease COVID-19. SARS …

The cellular composition of barrier epithelia is essential to organismal homoeostasis. In
particular, within the small intestine, adult stem cells establish tissue cellularity, and may …

Adult tissue-derived organoids allow for the expansion and maintenance of primary
epithelial cells in a near-native state. These 3D and self-organizing organotypic cultures …

Donor-to-donor variability in primary human organoid cultures has not been well
characterized. As these cultures contain multiple cell types, there is greater concern that …

Host defence peptides (HDPs), also known as antimicrobial peptides, are naturally occurring
polypeptides (~ 12-50 residues) composed of cationic and hydrophobic amino acids that …

Background & Aims Diligent side-by-side comparisons of how different methodologies affect
growth efficiency and quality of intestinal colonoids have not been performed leaving a gap …