In vivo two-photon calcium imaging (2PCI) is a technique used for recording neuronal
activity in the intact brain. It is based on the principle that, when neurons fire action …

Optogenetics ushered in a revolution in how neuroscientists interrogate brain function.
Because of technical limitations, the majority of optogenetic studies have used low spatial …

Neurons in cortical circuits are often coactivated as ensembles, yet it is unclear whether
ensembles play a functional role in behavior. Some ensemble neurons have pattern …

Recent progress in fluorescence imaging allows neuroscientists to observe the dynamics of
thousands of individual neurons, identified genetically or by their connectivity, across …

We present a modular approach for analyzing calcium imaging recordings of large neuronal
ensembles. Our goal is to simultaneously identify the locations of the neurons, demix …

The goal of computer-generated holography (CGH) is to synthesize custom illumination
patterns by modulating a coherent light beam. CGH algorithms typically rely on iterative …

Since the introduction of calcium imaging to monitor neuronal activity with single-cell
resolution, optical imaging methods have revolutionized neuroscience by enabling …

We describe an all-optical strategy for simultaneously manipulating and recording the
activity of multiple neurons with cellular resolution in vivo. We performed simultaneous two …

Understanding brain function requires technologies that can control the activity of large
populations of neurons with high fidelity in space and time. We developed a multiphoton …

There have been two recent revolutionary advances in neuroscience: First, genetically
encoded activity sensors have brought the goal of optical detection of single action …