We describe a new implementation for the reconstruction of helical assemblies in the
empirical Bayesian framework of RELION. Our approach calculates optimal linear filters for …

The function of actin is coupled to the nucleotide bound to its active site. ATP hydrolysis is
activated during polymerization; a delay between hydrolysis and inorganic phosphate (Pi) …

Regulation of myosin and filamentous actin interaction by tropomyosin is a central feature of
contractile events in muscle and nonmuscle cells. However, little is known about molecular …

Vinculin plays a fundamental role in integrin-mediated cell adhesion. Activated by talin, it
interacts with diverse adhesome components, enabling mechanical coupling between the …

Actin networks and actin-binding proteins (ABPs) are most abundant in the cytoskeleton of
neurons. The function of ABPs in neurons is nucleation of actin polymerization …

Single particle cryo-EM requires full automation to allow high-throughput structure
determination. Although software packages exist where parts of the cryo-EM pipeline are …

Kinetochores connect mitotic-spindle microtubules with chromosomes, allowing microtubule
depolymerization to pull chromosomes apart during anaphase while resisting detachment …

Molecular motors produce force when they interact with their cellular tracks. For myosin
motors, the primary force-generating state has MgADP tightly bound, whereas myosin is …

Myosin X has features not found in other myosins. Its structure must underlie its unique
ability to generate filopodia, which are essential for neuritogenesis, wound healing, cancer …

YiiP is a dimeric Zn2+/H+ antiporter from Escherichia coli belonging to the cation diffusion
facilitator family. We used cryoelectron microscopy to determine a 13-Å resolution structure …