The appropriate choice of reference genes is essential for accurate normalization of gene
expression data obtained by the method of reverse transcription quantitative real-time PCR …

Reference genes used in normalizing qRT-PCR data are critical for the accuracy of gene
expression analysis. However, many traditional reference genes used in zebrafish early …

Caseous lymphadenitis (LC) is a chronic contagious disease caused by Corynebacterium
pseudotuberculosis, which mainly affects goats and sheep. Vaccination is an effective but …

Reverse-transcription quantitative real-time polymerase chain reaction (RT-qPCR) using
fluorescent DNA-binding dyes is now a gold-standard methodology to study bacterial gene …

The human gut is inhabited by several hundred different bacterial species. These bacteria
are closely associated with our health and well-being. The composition of these diverse …

The Enterobacter cancerogenus strain EcHa1 was isolated from the dead larvae of
Helicoverpa armigera, and has the potential for biocontrol of some Lepidoptera insects. In …

Quantitative real-time polymerase chain reaction is a powerful tool for quantifying gene
expression. The relative quantification relies on normalizing the data to reference genes or …

RNA sequencing (RNA-Seq)-based gene expression analysis is applicable to a wide range
of biological purposes in various species. Reverse transcription quantitative PCR (RT …

Corynebacterium pseudotuberculosis strain VD57 (Cp_VD57), a highly virulent, nonmotile,
non-sporulating, and a mesophilic bacterium, was isolated from a goat's granulomatous …

Clavibacter michiganensis, the causal agent of bacterial canker of tomato, is a Gram‐
positive bacterium and a model for studying plant diseases. The real‐time quantitative …