Quantitative characterization of recombinase-based digitizer circuits enables predictable amplification of biological signals

KA Kiwimagi, JH Letendre, BH Weinberg… - Communications …, 2021 - nature.com
Many synthetic gene circuits are restricted to single-use applications or require iterative
refinement for incorporation into complex systems. One example is the recombinase-based …

How retroactivity affects the behavior of incoherent feedforward loops

J Wang, C Belta, SA Isaacson - Iscience, 2020 - cell.com
An incoherent feedforward loop (IFFL) is a network motif known for its ability to accelerate
responses and generate pulses. It remains an open question to understand the behavior of …

Modeling genetic circuit behavior in transiently transfected mammalian cells

J Wang, SA Isaacson, C Belta - ACS synthetic biology, 2019 - ACS Publications
Binning cells by plasmid copy number is a common practice for analyzing transient
transfection data. In many kinetic models of transfected cells, protein production rates are …

Genetic Circuit Designs to Improve Synthetic Biological Signaling in Mammalian Cells

JH Letendre - 2022 - search.proquest.com
Multicellular organisms perform sophisticated computations on a myriad of environmental
cues and produce many discrete cell states in response. These phenotypes are often …