Enzymatic processing of lignocellulosic biomass: principles, recent advances and perspectives

H Østby, LD Hansen, SJ Horn… - Journal of Industrial …, 2020 - academic.oup.com
Efficient saccharification of lignocellulosic biomass requires concerted development of a
pretreatment method, an enzyme cocktail and an enzymatic process, all of which are …

Engineering strategies for enhanced production of protein and bio-products in Pichia pastoris: a review

Z Yang, Z Zhang - Biotechnology advances, 2018 - Elsevier
Pichia pastoris has been recognized as one of the most industrially important hosts for
heterologous protein production. Despite its high protein productivity, the optimization of P …

Oxidative cleavage of polysaccharides by monocopper enzymes depends on H2O2

B Bissaro, ÅK Røhr, G Müller, P Chylenski… - Nature chemical …, 2017 - nature.com
Enzymes currently known as lytic polysaccharide monooxygenases (LPMOs) play an
important role in the conversion of recalcitrant polysaccharides, but their mode of action has …

Lytic xylan oxidases from wood-decay fungi unlock biomass degradation

M Couturier, S Ladeveze, G Sulzenbacher… - Nature chemical …, 2018 - nature.com
Wood biomass is the most abundant feedstock envisioned for the development of modern
biorefineries. However, the cost-effective conversion of this form of biomass into commodity …

Oxidoreductases and reactive oxygen species in conversion of lignocellulosic biomass

B Bissaro, A Várnai, ÅK Røhr… - … and Molecular Biology …, 2018 - Am Soc Microbiol
Biomass constitutes an appealing alternative to fossil resources for the production of
materials and energy. The abundance and attractiveness of vegetal biomass come along …

Microbial lignin valorization through depolymerization to aromatics conversion

F Li, Y Zhao, L Xue, F Ma, SY Dai, S Xie - Trends in biotechnology, 2022 - cell.com
Lignin is the most abundant source of renewable aromatic biopolymers and its valorization
presents significant value for biorefinery sustainability, which promotes the utilization of …

Investigating lytic polysaccharide monooxygenase-assisted wood cell wall degradation with microsensors

H Chang, N Gacias Amengual, A Botz… - Nature …, 2022 - nature.com
Lytic polysaccharide monooxygenase (LPMO) supports biomass hydrolysis by increasing
saccharification efficiency and rate. Recent studies demonstrate that H2O2 rather than O2 is …

A conserved second sphere residue tunes copper site reactivity in lytic polysaccharide monooxygenases

KR Hall, C Joseph, I Ayuso-Fernández… - Journal of the …, 2023 - ACS Publications
Lytic polysaccharide monooxygenases (LPMOs) are powerful monocopper enzymes that
can activate strong C–H bonds through a mechanism that remains largely unknown. Herein …

Discovery of LPMO activity on hemicelluloses shows the importance of oxidative processes in plant cell wall degradation

JW Agger, T Isaksen, A Várnai… - Proceedings of the …, 2014 - National Acad Sciences
The recently discovered lytic polysaccharide monooxygenases (LPMOs) are known to carry
out oxidative cleavage of glycoside bonds in chitin and cellulose, thus boosting the activity of …

Lytic polysaccharide monooxygenases: enzymes for controlled and site-specific Fenton-like chemistry

B Bissaro, VGH Eijsink - Essays in biochemistry, 2023 - portlandpress.com
The discovery of oxidative cleavage of glycosidic bonds by enzymes currently known as lytic
polysaccharide monooxygenases (LPMOs) has profoundly changed our current …