Studying cell metabolism serves a plethora of objectives such as the enhancement of
bioprocess performance, and advancement in the understanding of cell biology, of drug …

Metabolic networks are regulated to ensure the dynamic adaptation of biochemical reaction
fluxes to maintain cell homeostasis and optimal metabolic fitness in response to …

Pseudomonas putida is evolutionarily endowed with features relevant for bioproduction,
especially under harsh operating conditions. The rich metabolic versatility of this species …

Genome-scale metabolic networks (GSMs) are fundamental systems biology
representations of a cell's entire set of stoichiometrically balanced reactions. However, such …

Generating large omics datasets has become routine for gaining insights into cellular
processes, yet deciphering these datasets to determine metabolic states remains …

Highlights•Kinetic models integrate heterogeneous data to give metabolic engineering
insights.•Need accurate catalytic and allostery kinetic parameters for quality kinetic …

Metabolic flux analysis (MFA) quantitatively describes cellular fluxes to understand
metabolic phenotypes and functional behaviour after environmental and/or genetic …

The parameterization of kinetic models requires measurement of fluxes and/or metabolite
levels for a base strain and a few genetic perturbations thereof. Unlike stoichiometric models …

Understanding the governing principles behind organisms' metabolism and growth
underpins their effective deployment as bioproduction chassis. A central objective of …

Cell-free systems are useful tools for prototyping metabolic pathways and optimizing the
production of various bioproducts. Mechanistically-based kinetic models are uniquely suited …