Isothermal titration calorimetry (ITC) is a fast and robust method to determine the energetics
of association reactions in solution. The changes in enthalpy, entropy and heat capacity that …

Intrinsically disordered proteins (IDPs) and proteins with long intrinsically disordered protein
regions (IDPRs) lack ordered structure but are involved in a multitude of biological …

Herpesvirus genomes are maintained as extrachromosomal plasmids within the nuclei of
infected cells. Some herpesviruses persist within dividing cells, putting the viral genome at …

Neuronal specification is regulated by the activity of transcription factors containing the basic
helix− loop− helix motif (bHLH); these regulating proteins include, among others, the …

Mucosal human papillomaviruses (HPVs) are etiological agents of oral, anal and genital
cancer. Properties of high-and low-risk HPV types cannot be reduced to discrete molecular …

We describe the formation of protein–DNA contacts in the two-state route for DNA sequence
recognition by a transcriptional regulator. Surprisingly, direct sequence readout establishes …

Papillomavirus E2 proteins play a central role in regulating viral gene expression and
replication. DNA-binding activity is associated with the C-terminal domain of E2, which forms …

The DNA binding domain of papillomavirus E2 proteins is at the center of the regulation of
gene transcription and replication of the virus. Its unique fold consists of a β‐barrel domain …

The E2 proteins are transcription/replication factors from papillomaviruses. Human
papillomaviruses (HPVs) can be broadly divided in two groups; low-risk HPV subtypes …

Although DNA flexibility is known to play an important role in DNA–protein interactions, the
importance of protein flexibility is less well understood. Here, we show that protein dynamics …