Evolutionary mining and functional characterization of TnpB nucleases identify efficient miniature genome editors
G Xiang, Y Li, J Sun, Y Huo, S Cao, Y Cao, Y Guo… - Nature …, 2024 - nature.com
As the evolutionary ancestor of Cas12 nuclease, the transposon (IS200/IS605)-encoded
TnpB proteins act as compact RNA-guided DNA endonucleases. To explore their …
TnpB proteins act as compact RNA-guided DNA endonucleases. To explore their …
The never-ending battle between lactic acid bacteria and their phages
Over the past few decades, the interest in lactic acid bacteria (LAB) has been steadily
growing. This is mainly due to their industrial use, their health benefits as probiotic bacteria …
growing. This is mainly due to their industrial use, their health benefits as probiotic bacteria …
Meta-analysis of cheese microbiomes highlights contributions to multiple aspects of quality
A detailed understanding of the cheese microbiome is key to the optimization of flavour,
appearance, quality and safety. Accordingly, we conducted a high-resolution meta-analysis …
appearance, quality and safety. Accordingly, we conducted a high-resolution meta-analysis …
The phage defence island of a multidrug resistant plasmid uses both BREX and type IV restriction for complementary protection from viruses
DM Picton, YA Luyten, RD Morgan… - Nucleic acids …, 2021 - academic.oup.com
Bacteria have evolved a multitude of systems to prevent invasion by bacteriophages and
other mobile genetic elements. Comparative genomics suggests that genes encoding …
other mobile genetic elements. Comparative genomics suggests that genes encoding …
dbAPIS: a database of a nti-p rokaryotic i mmune s ystem genes
Anti-prokaryotic immune system (APIS) proteins, typically encoded by phages, prophages,
and plasmids, inhibit prokaryotic immune systems (eg restriction modification, toxin-antitoxin …
and plasmids, inhibit prokaryotic immune systems (eg restriction modification, toxin-antitoxin …
[HTML][HTML] In silico approaches for prediction of anti-CRISPR proteins
KS Makarova, YI Wolf, EV Koonin - Journal of molecular biology, 2023 - Elsevier
Numerous viruses infecting bacteria and archaea encode CRISPR-Cas system inhibitors,
known as anti-CRISPR proteins (Acr). The Acrs typically are highly specific for particular …
known as anti-CRISPR proteins (Acr). The Acrs typically are highly specific for particular …
Widespread CRISPR-derived RNA regulatory elements in CRISPR-Cas systems
CRISPR-cas loci typically contain CRISPR arrays with unique spacers separating direct
repeats. Spacers along with portions of adjacent repeats are transcribed and processed into …
repeats. Spacers along with portions of adjacent repeats are transcribed and processed into …
Prophage integration into CRISPR loci enables evasion of antiviral immunity in Streptococcus pyogenes
CRISPR loci are composed of short DNA repeats separated by sequences, known as
spacers, that match the genomes of invaders such as phages and plasmids. Spacers are …
spacers, that match the genomes of invaders such as phages and plasmids. Spacers are …
Widespread repression of anti-CRISPR production by anti-CRISPR-associated proteins
Many bacteria use CRISPR-Cas systems to defend against invasive mobile genetic
elements (MGEs). In response, MGEs have developed strategies to resist CRISPR-Cas …
elements (MGEs). In response, MGEs have developed strategies to resist CRISPR-Cas …
AcrFinder: genome mining anti-CRISPR operons in prokaryotes and their viruses
Anti-CRISPR (Acr) proteins encoded by (pro) phages/(pro) viruses have a great potential to
enable a more controllable genome editing. However, genome mining new Acr proteins is …
enable a more controllable genome editing. However, genome mining new Acr proteins is …