▪ Abstract The compartmentation of eukaryotic cells requires all nuclear proteins to be
imported from the cytoplasm, whereas, for example, transfer RNAs, messenger RNAs, and …

▪ Abstract The synthesis of ribosomes is one of the major metabolic pathways in all cells. In
addition to around 75 individual ribosomal proteins and 4 ribosomal RNAs, synthesis of a …

The importin‐alpha/beta heterodimer and the GTPase Ran play key roles in nuclear protein
import. Importin binds the nuclear localization signal (NLS). Translocation of the resulting …

We have combined techniques of genetics and histochemistry to identify genes required for
the nucleocytoplasmic export of mRNA in the budding yeast Saccharomyces cerevisiae. We …

The defining feature of eukaryotic organisms is the cell nucleus. All nuclear proteins are
synthesized in the cytoplasm and need to be imported through the nuclear pore complexes …

RanGAP1 is the GTPase activator for the nuclear Ras-related regulatory protein Ran,
converting it to the putatively inactive GDP-bound state. Here, we report the amino acid …

Post‐transcriptional processing of precursor‐ribosomal RNA comprises a complex pathway
of endonucleolytic cleavages, exonucleolytic digestion and covalent modifications. The …

The response of eukaryotic cells to heat shock and other forms of stress occurs at both
transcriptional and post-transcriptional levels. We used in situ hybridization to determine …

The yeast RNA1 gene is required for RNA processing and nuclear transport of RNA. The
rna1-1 mutation of this locus causes defects in pre-tRNA splicing, processing of the primary …

Importin-α mediates nuclear protein import by binding nuclear localization signals and
importin-β. We find approximately 30% of SRP1p, the yeast importin-α, in a nuclear complex …