Single‐cell RNA sequencing (scRNA‐seq) technology has become the state‐of‐the‐art
approach for unravelling the heterogeneity and complexity of RNA transcripts within …

The generation of islet-like endocrine clusters from human pluripotent stem cells (hPSCs)
has the potential to provide an unlimited source of insulin-producing β cells for the treatment …

We designed a unique nanocapsule for efficient single CRISPR-Cas9 capsuling,
noninvasive brain delivery and tumor cell targeting, demonstrating an effective and safe …

Genetic engineering of allogeneic cell therapeutics that fully prevents rejection by a
recipient's immune system would abolish the requirement for immunosuppressive drugs or …

Recent advances in genome editing technologies have magnified the prospect of single-
dose cures for many genetic diseases. For most genetic disorders, precise DNA correction is …

We detail a six-stage planar differentiation methodology for generating human pluripotent
stem cell–derived pancreatic β cells (SC-β cells) that secrete high amounts of insulin in …

Insulin-producing β cells created from human pluripotent stem cells have potential as a
therapy for insulin-dependent diabetes, but human pluripotent stem cell-derived islets (SC …

Diabetes mellitus is a metabolic disorder that affects more than 460 million people
worldwide. Type 1 diabetes (T1D) is caused by autoimmune destruction of β-cells, whereas …

CRISPR-Cas technology has revolutionized biological research and holds great therapeutic
potential. Here, we review CRISPR-Cas systems and their latest developments with an …

Human pluripotent stem cells differentiated to insulin-secreting β cells (SC-β cells) in islet
organoids could provide an unlimited cell source for diabetes cell replacement therapy …