Ribosomal protein genes are among the most highly expressed genes in most cell types.
Their products are generally essential for ribosome synthesis, which is the cornerstone for …

In this review, we discuss transposon-insertion sequencing, variously known in the literature
as TraDIS, Tn-seq, INSeq, and HITS. By monitoring a large library of single transposon …

Many functional RNAs have an evolutionarily conserved secondary structure. Conservation
of RNA base pairing induces pairwise covariations in sequence alignments. We developed …

Summary mRNAs can fold into complex structures that regulate gene expression. Resolving
such structures de novo has remained challenging and has limited our understanding of the …

Summary 7SK is a conserved noncoding RNA that regulates transcription by sequestering
the transcription factor P-TEFb. 7SK function entails complex changes in RNA structure, but …

RNA structure and dynamics are critical to biological function. However, strategies for
determining RNA structure in vivo are limited, with established chemical probing and newer …

Ever since its discovery, the double-stranded DNA contained in the mitochondria of
eukaryotes has fascinated researchers because of its bacterial endosymbiotic origin, crucial …

Metagenome-assembled genomes (MAGs) have revealed the hidden diversity and functions
of uncultivated microbes, but their reconstruction from metagenomes remains a …

To adapt their metabolism rapidly and constantly in response to environmental variations,
bacteria often target the translation initiation process, during which the ribosome assembles …

Nucleotides are at the heart of the most essential biological processes in the cell, be it as
key protagonists in the dogma of molecular biology or by regulating multiple metabolic …