Agrobacterium strains transfer a single-strand form of T-DNA (T-strands) and Virulence (Vir)
effector proteins to plant cells. Following transfer, T-strands likely form complexes with Vir …

With the emergence of high‐throughput methods in plant biology, the importance of long‐
term projects characterized by incremental advances involving multiple laboratories can …

Targeted mutagenesis is an essential tool of reverse genetics that could be used
experimentally to investigate basic plant biology or modify crop plants for improvement of …

The testing and release of genetically modified organisms (GMOs)—in particular GM plants—
is tightly regulated internationally to prevent any negative effects on the environment or …

Background Going from a gene sequence to its function in the context of a whole organism
requires a strategy for targeting mutations, referred to as reverse genetics. Reverse genetics …

In the present genomics era, powerful reverse-genetic strategies are needed to elucidate
gene and protein function in the context of a whole organism. However, most current …

Background Stresses exert evolutionary pressures on all organisms, which have developed
sophisticated responses to cope and survive. These responses involve cellular physiology …

Analysis of the genome sequence of Arabidopsis thaliana, as well as that of all other
organisms for which whole-genome sequences are available, has revealed the existence of …

To analyze relationships between RNA signals, DNA methylation and chromatin
modifications, we performed a genetic screen to recover Arabidopsis mutants defective in …

Background Experimental proof of gene function assignments in plants is based on mutant
analyses. T-DNA insertion lines provided an invaluable resource of mutants and enabled …