The removal of introns from mRNA precursors and its regulation by alternative splicing are
key for eukaryotic gene expression and cellular function, as evidenced by the numerous …

Nuclear speckles are dynamic membraneless bodies located in the cell nucleus. They
harbor RNAs and proteins, many of which are splicing factors, that together display complex …

The nucleus is highly compartmentalized through the formation of distinct classes of
membraneless domains. However, the composition and function of many of these structures …

Long introns with short exons in vertebrate genes are thought to require spliceosome
assembly across exons (exon definition), rather than introns, thereby requiring transcription …

Nuclear speckles are nuclear membraneless organelles in higher eukaryotic cells playing a
vital role in gene expression. Using an in situ reverse transcription–based sequencing …

Many steps of RNA processing occur during transcription by RNA polymerases. Co-
transcriptional activities are deemed commonplace in prokaryotes, in which the lack of …

The nucleus is highly organized, such that factors involved in the transcription and
processing of distinct classes of RNA are confined within specific nuclear bodies,. One …

Histones shape chromatin structure and the epigenetic landscape. H1, the most diverse
histone in the human genome, has 11 variants. Due to the high structural similarity between …

Splicing of pre-mRNAs critically contributes to gene regulation and proteome expansion in
eukaryotes, but our understanding of the recognition and pairing of splice sites during …

The spliceosome is the complex molecular machinery that sequentially assembles on
eukaryotic messenger RNA precursors to remove introns (pre-mRNA splicing), a …