SARS-CoV-2 enters host cells through an interaction between the spike glycoprotein and
the angiotensin converting enzyme 2 (ACE2) receptor. Directly preventing this interaction …

Targeted PCR amplification and high-throughput sequencing (amplicon sequencing) of 16S
rRNA gene fragments is widely used to profile microbial communities. New long-read …

Conventional immunization strategies will likely be insufficient for the development of a
broadly neutralizing antibody (bnAb) vaccine for HIV or other difficult pathogens because of …

Background Out of the many pathogenic bacterial species that are known, only a fraction are
readily identifiable directly from a complex microbial community using standard next …

Definition of the specific germline immunoglobulin (Ig) alleles present in an individual is a
critical first step to delineate the ontogeny and evolution of antigen-specific antibody …

Quantifying the replication-competent HIV reservoir is essential for evaluating curative
strategies. Viral outgrowth assays (VOAs) underestimate the reservoir because they fail to …

HIV broadly neutralizing antibodies (bnAbs) can suppress viremia and protect against HIV
infection. However, their elicitation is made difficult by low frequencies of appropriate …

The continued evolution of SARS-CoV-2 underscores the need to understand qualitative
aspects of the humoral immune response elicited by spike immunization. Here, we combine …

Conventional approaches to isolate and characterize nanobodies are laborious. We
combine phage display, multivariate enrichment, next-generation sequencing, and a …

The membrane-proximal external region (MPER) of HIV-1 envelope glycoprotein (Env) can
be targeted by neutralizing antibodies of exceptional breadth. MPER antibodies usually …