Next-generation sequencing technology: current trends and advancements

H Satam, K Joshi, U Mangrolia, S Waghoo, G Zaidi… - Biology, 2023 - mdpi.com
Simple Summary Next-generation sequencing (NGS) is a powerful tool used in genomics
research. NGS can sequence millions of DNA fragments at once, providing detailed …

Shotgun metagenomics, from sampling to analysis

C Quince, AW Walker, JT Simpson, NJ Loman… - Nature …, 2017 - nature.com
Diverse microbial communities of bacteria, archaea, viruses and single-celled eukaryotes
have crucial roles in the environment and in human health. However, microbes are …

SKESA: strategic k-mer extension for scrupulous assemblies

A Souvorov, R Agarwala, DJ Lipman - Genome biology, 2018 - Springer
SKESA is a DeBruijn graph-based de-novo assembler designed for assembling reads of
microbial genomes sequenced using Illumina. Comparison with SPAdes and MegaHit …

metaSPAdes: a new versatile metagenomic assembler

S Nurk, D Meleshko, A Korobeynikov… - Genome …, 2017 - genome.cshlp.org
While metagenomics has emerged as a technology of choice for analyzing bacterial
populations, the assembly of metagenomic data remains challenging, thus stifling biological …

MEGAHIT v1. 0: a fast and scalable metagenome assembler driven by advanced methodologies and community practices

D Li, R Luo, CM Liu, CM Leung, HF Ting, K Sadakane… - Methods, 2016 - Elsevier
The study of metagenomics has been much benefited from low-cost and high-throughput
sequencing technologies, yet the tremendous amount of data generated make analysis like …

IDBA-UD: a de novo assembler for single-cell and metagenomic sequencing data with highly uneven depth

Y Peng, HCM Leung, SM Yiu, FYL Chin - Bioinformatics, 2012 - academic.oup.com
Motivation: Next-generation sequencing allows us to sequence reads from a microbial
environment using single-cell sequencing or metagenomic sequencing technologies …

[HTML][HTML] The role of the gut microbiome in the healthy adult status

V D'Argenio, F Salvatore - Clinica chimica acta, 2015 - Elsevier
The gut microbiome, which hosts up to 1000 bacterial species that encode about 5 million
genes, perform many of the functions required for host physiology and survival …

MaxBin: an automated binning method to recover individual genomes from metagenomes using an expectation-maximization algorithm

YW Wu, YH Tang, SG Tringe, BA Simmons, SW Singer - Microbiome, 2014 - Springer
Background Recovering individual genomes from metagenomic datasets allows access to
uncultivated microbial populations that may have important roles in natural and engineered …

An introduction to the analysis of shotgun metagenomic data

TJ Sharpton - Frontiers in plant science, 2014 - frontiersin.org
Environmental DNA sequencing has revealed the expansive biodiversity of microorganisms
and clarified the relationship between host-associated microbial communities and host …

Metagenomics-a guide from sampling to data analysis

T Thomas, J Gilbert, F Meyer - Microbial informatics and experimentation, 2012 - Springer
Metagenomics applies a suite of genomic technologies and bioinformatics tools to directly
access the genetic content of entire communities of organisms. The field of metagenomics …