A CRISPR interference screen of essential genes reveals that proteasome regulation dictates acetic acid tolerance in Saccharomyces cerevisiae

V Mukherjee, U Lind, RP St. Onge, A Blomberg… - Msystems, 2021 - Am Soc Microbiol
ABSTRACT CRISPR interference (CRISPRi) is a powerful tool to study cellular physiology
under different growth conditions, and this technology provides a means for screening …

A high-throughput method for screening for genes controlling bacterial conjugation of antibiotic resistance

H Alalam, FE Graf, M Palm, M Abadikhah… - Msystems, 2020 - Am Soc Microbiol
The rapid horizontal transmission of antibiotic resistance genes on conjugative plasmids
between bacterial host cells is a major cause of the accelerating antibiotic resistance crisis …

Evolution of microbial growth traits under serial dilution

J Lin, M Manhart, A Amir - Genetics, 2020 - academic.oup.com
Selection of mutants in a microbial population depends on multiple cellular traits. In serial-
dilution evolution experiments, three key traits are the lag time when transitioning from …

Machine learning prediction of resistance to subinhibitory antimicrobial concentrations from Escherichia coli genomes

S Benkwitz-Bedford, M Palm, TY Demirtas… - Msystems, 2021 - Am Soc Microbiol
Escherichia coli is an important cause of bacterial infections worldwide, with multidrug-
resistant strains incurring substantial costs on human lives. Besides therapeutic …

Synthetic physical interactions with the yeast centrosome

RSM Howell, A Csikász-Nagy… - G3: Genes, Genomes …, 2019 - academic.oup.com
The yeast centrosome or Spindle Pole Body (SPB) is an organelle situated in the nuclear
membrane, where it nucleates spindle microtubules and acts as a signaling hub. Various …

Lactose-assimilating yeasts with high fatty acid accumulation uncovered by untargeted bioprospecting

C Geijer, K Persson, KVR Peri, J Warringer, A Moneke… - 2024 - researchsquare.com
Bioprospecting can uncover new yeast strains and species with valuable biotechnological
and industrial traits, such as the capacity to convert different carbon sources in industrial …

Genome-wide dynamic evaluation of the UV-induced DNA damage response

E Silva, M Michaca, B Munson, GJ Bean… - G3: Genes …, 2020 - academic.oup.com
Genetic screens in Saccharomyces cerevisiae have allowed for the identification of many
genes as sensors or effectors of DNA damage, typically by comparing the fitness of genetic …

[PDF][PDF] A High-Throughput Method for Screening for Genes Controlling Bacterial Conjugation of Antibiotic Resistance

C Hadjineophytou, L Persson, S Stenberg, M Mattsson… - 2020 - academia.edu
The rapid horizontal transmission of antibiotic resistance genes on conjugative plasmids
between bacterial host cells is a major cause of the accelerating antibiotic resistance crisis …