[HTML][HTML] High-content CRISPR screening
CRISPR screens are a powerful source of biological discovery, enabling the unbiased
interrogation of gene function in a wide range of applications and species. In pooled …
interrogation of gene function in a wide range of applications and species. In pooled …
The promise and challenge of therapeutic genome editing
JA Doudna - Nature, 2020 - nature.com
Genome editing, which involves the precise manipulation of cellular DNA sequences to alter
cell fates and organism traits, has the potential to both improve our understanding of human …
cell fates and organism traits, has the potential to both improve our understanding of human …
Efficient engineering of human and mouse primary cells using peptide-assisted genome editing
Simple, efficient and well-tolerated delivery of CRISPR genome editing systems into primary
cells remains a major challenge. Here we describe an engineered Peptide-Assisted …
cells remains a major challenge. Here we describe an engineered Peptide-Assisted …
In vivo CD8+ T cell CRISPR screening reveals control by Fli1 in infection and cancer
Improving effector activity of antigen-specific T cells is a major goal in cancer
immunotherapy. Despite the identification of several effector T cell (T EFF)-driving …
immunotherapy. Despite the identification of several effector T cell (T EFF)-driving …
The interferon-stimulated gene RIPK1 regulates cancer cell intrinsic and extrinsic resistance to immune checkpoint blockade
L Cucolo, Q Chen, J Qiu, Y Yu, M Klapholz… - Immunity, 2022 - cell.com
Summary Interferon-gamma (IFN-γ) has pleiotropic effects on cancer immune checkpoint
blockade (ICB), including roles in ICB resistance. We analyzed gene expression in ICB …
blockade (ICB), including roles in ICB resistance. We analyzed gene expression in ICB …
The SWI/SNF chromatin remodeling complexes BAF and PBAF differentially regulate epigenetic transitions in exhausted CD8+ T cells
CD8+ T cell exhaustion (Tex) limits disease control during chronic viral infections and
cancer. Here, we investigated the epigenetic factors mediating major chromatin-remodeling …
cancer. Here, we investigated the epigenetic factors mediating major chromatin-remodeling …
Titrating gene expression using libraries of systematically attenuated CRISPR guide RNAs
A lack of tools to precisely control gene expression has limited our ability to evaluate
relationships between expression levels and phenotypes. Here, we describe an approach to …
relationships between expression levels and phenotypes. Here, we describe an approach to …
Small-molecule inhibition of the acyl-lysine reader ENL as a strategy against acute myeloid leukemia
The chromatin reader eleven–nineteen leukemia (ENL) has been identified as a critical
dependency in acute myeloid leukemia (AML), but its therapeutic potential remains unclear …
dependency in acute myeloid leukemia (AML), but its therapeutic potential remains unclear …
HIC2 controls developmental hemoglobin switching by repressing BCL11A transcription
The fetal-to-adult switch in hemoglobin production is a model of developmental gene control
with relevance to the treatment of hemoglobinopathies. The expression of transcription factor …
with relevance to the treatment of hemoglobinopathies. The expression of transcription factor …
Systematic discovery and perturbation of regulatory genes in human T cells reveals the architecture of immune networks
Gene regulatory networks ensure that important genes are expressed at precise levels.
When gene expression is sufficiently perturbed, it can lead to disease. To understand how …
When gene expression is sufficiently perturbed, it can lead to disease. To understand how …