High-throughput sequencing (HTS) is becoming the state-of-the-art technology for typing of
microbial isolates, especially in clinical samples. Yet, its application is still in its infancy for …

Genomics, transcriptomics, and proteomics are rapidly transforming our approaches to the
detection, prevention, and treatment of foodborne pathogens. Microbial genome sequencing …

Noroviruses are the most frequent cause of epidemic acute gastroenteritis in the United
States. Between September 2013 and August 2016, 2,715 genotyped norovirus outbreaks …

The term 'shotgun metagenomics' is applied to the direct sequencing of DNA extracted from
a sample without culture or target-specific amplification or capture. In diagnostic …

Abstract The development of High-Throughput Sequencing (HTS) technologies is having a
major impact on the genomic analysis of viral populations. Current HTS platforms can …

Norovirus full-genome sequencing is challenging due to sequence heterogeneity among
genomes. Previous methods have relied on PCR amplification, which is problematic due to …

Abstract Background Next-Generation Sequencing (NGS) is revolutionizing molecular
epidemiology by providing new approaches to undertake whole genome sequencing (WGS) …

Norovirus is estimated to cause 677 million annual cases of gastroenteritis worldwide,
resulting in 210,000 deaths. As viral gastroenteritis is generally self-limiting, clinical samples …

For the past two decades, norovirus pandemic variants have emerged every 3–5 years, and
dominate until they are replaced by alternate strains. However, this scenario changed in …

The control of re-occurring pandemic pathogens requires understanding the origins of new
pandemic variants and the factors that drive their global spread. This is especially important …