The Chilean soapbark tree (Quillaja saponaria) produces soap-like molecules called QS
saponins that are important vaccine adjuvants. These highly valuable compounds are …

Regulatory bodies worldwide consider N-glycosylation to be a critical quality attribute for
immunoglobulin G (IgG) and IgG-like therapeutics. This consideration is due to the …

Highlights•Nucleotide sugars are precursors to the sugars involved in protein N-
glycosylation.•Different media supplements, such as N-acetylmannosamine and uridine …

Glycosylation of biomolecules can greatly alter their physicochemical properties, cellular
recognition, subcellular localization, and immunogenicity. Glycosylation reactions rely on the …

Glycoside phosphorylases (EC 2.4. xx) carry out the reversible phosphorolysis of glucan
polymers, producing the corresponding sugar 1-phosphate and a shortened glycan chain. β …

Metabolic modelling strives to develop modelling approaches that are robust and highly
predictive. To achieve this, various modelling designs, including hybrid models, and …

The analysis of biological samples is often affected by the background matrix. Proper
sample preparation is a critical step in the analytical procedure for complex samples. In this …

Euglena gracilis is an alga of great biotechnological interest and extensive metabolic
capacity, able to make high levels of bioactive compounds, such as polyunsaturated fatty …

Small molecule monosaccharide analogs (eg 4F-GlcNAc, 4F-GalNAc) and acceptor decoys
(eg ONAP, SNAP) are commonly used as metabolic glycoengineering tools to perturb …

Nucleotides and their sugar conjugates are fundamental molecules in life, participating in
processes of DNA/RNA composition, cell wall build-up, glycosylation reactions, and signal …