The yeast two-hybrid system pioneered the field of in vivo protein-protein interaction
methods and undisputedly gave rise to a palette of ingenious techniques that are constantly …

Protein-protein interactions (PPIs) are essential components of cellular function. Current
fluorescence-based technologies to measure PPIs have limited dynamic range and …

Infection with many mammalian orthoreovirus (MRV) strains results in shutoff of host, but not
viral, protein synthesis via protein kinase R (PKR) activation and phosphorylation of …

Genetic high throughput screens have yielded large sets of potential protein-protein
interactions now to be verified and further investigated. Here we present a simple assay to …

Virally induced structures called viral factories form throughout the cytoplasm of cells
infected with mammalian orthoreoviruses (MRV). When expressed alone in cells, MRV …

Rotaviruses (RVs) are classified into nine species, A–D and F–J, with species A being the
most studied. In rotavirus of species A (RVA), replication occurs in viroplasms, which are …

Within infected host cells, mammalian orthoreovirus (MRV) forms viral factories (VFs), which
are sites of viral transcription, translation, assembly, and replication. The MRV nonstructural …

Mammalian orthoreovirus (MRV) infection induces phosphorylation of translation initiation
factor eIF2α, which promotes the formation of discrete cytoplasmic inclusions, termed stress …

Expression of a high-risk human papillomavirus (HPV) E7 oncoprotein is sufficient to induce
aberrant centrosome duplication in primary human cells. The resulting centrosome …

Using genetic approaches, particle image velocimetry and an inert tracer of cytoplasmic
streaming, we have made a mechanistic connection between the motor proteins (myosins …