Single domain antibody: Development and application in biotechnology and biopharma

T Yu, F Zheng, W He, S Muyldermans… - Immunological …, 2024 - Wiley Online Library
Heavy‐chain antibodies (HCAbs) are a unique type of antibodies devoid of light chains, and
comprised of two heavy chains‐only that recognize their cognate antigen by virtue of a …

Microbial single-cell applications under anoxic conditions

C Keating, K Fiege, M Diender, DZ Sousa… - Applied and …, 2024 - journals.asm.org
The field of microbiology traditionally focuses on studying microorganisms at the population
level. Nevertheless, the application of single-cell level methods, including microfluidics and …

The potential of ALFA-tag and tyramide-based fluorescence signal amplification to expand the CRISPR-based DNA imaging toolkit

BP Potlapalli, J Fuchs, T Rutten… - Journal of …, 2024 - academic.oup.com
Understanding the spatial organization of genomes within chromatin is crucial for
deciphering gene regulation. A recently developed CRISPR-dCas9-based genome labeling …

[HTML][HTML] EpicTope: narrating protein sequence features to identify non-disruptive epitope tagging sites

J Zinski, H Chung, P Joshi, F Warrick, BD Berg… - bioRxiv, 2024 - ncbi.nlm.nih.gov
Epitope tagging is an invaluable technique enabling the identification, tracking, and
purification of proteins in vivo. We developed a tool, EpicTope, to facilitate this method by …

The Z-Ring in Multicellular Cyanobacteria has a dynamic pearl necklace arrangement

M Vásquez, J Olivares, DA Molina, A González-Crespo… - 2024 - researchsquare.com
Z-ring formation by FtsZ in the midcell is a key event in bacterial cell division. Results
obtained with different super-resolution techniques have shown that the Z-ring is …