Next-generation sequencing allows the analysis of genomes, including those representing
disease states. However, the causes of most disorders are multifactorial, and systems-level …

Quantitative measurement of proteins is one of the most fundamental analytical tasks in a
biochemistry laboratory, but widely used immunochemical methods often have limited …

The human cerebral cortex houses 1000 times more neurons than that of the cerebral cortex
of a mouse, but the possible differences in synaptic circuits between these species are still …

Isobaric labeling empowers proteome-wide expression measurements simultaneously
across multiple samples. Here an expanded set of 16 isobaric reagents based on an …

The molecular circuits by which antigens activate quiescent T cells remain poorly
understood. We combined temporal profiling of the whole proteome and phosphoproteome …

We describe the comprehensive analysis of the yeast proteome in just over one hour of
optimized analysis. We achieve this expedited proteome characterization with improved …

Calorie restriction (CR) extends life span in diverse species. Mitochondria play a key role in
CR adaptation; however, the molecular details remain elusive. We developed and applied a …

Sleep and wake have global effects on brain physiology, from molecular changes,,–and
neuronal activities to synaptic plasticity,,,–. Sleep–wake homeostasis is maintained by the …

Spermatozoa must leave one organism, navigate long distances, and deliver their paternal
DNA into a mature egg. For successful navigation and delivery, a sperm-specific calcium …

The reversible phosphorylation of proteins on serine, threonine, and tyrosine residues is an
important biological regulatory mechanism. In the context of genome integrity, signaling …