Considerable progress towards an understanding of complex diseases has been made in
recent years due to the development of high-throughput genotyping technologies. Using …

The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system, a
simple and efficient tool for genome editing, has experienced rapid progress in its …

The targeted deletion, replacement, integration or inversion of genomic sequences could be
used to study or treat human genetic diseases, but existing methods typically require double …

CTCF and the associated cohesin complex play a central role in insulator function and
higher-order chromatin organization of mammalian genomes. Recent studies identified a …

Although CRISPR/Cas9 genome editing has provided numerous opportunities to interrogate
the functional significance of any given genomic site, there is a paucity of data on the extent …

Structural variations (SVs), such as inversion and duplication, contribute to important
agronomic traits in crops. Pan-genome studies revealed that SVs were a crucial and …

Fusion oncogenes (FOs) are common in many cancer types and are powerful drivers of
tumor development. Because their expression is exclusive to cancer cells and their …

Abstract The CRISPR/Cas9-sgRNA system has been developed to mediate genome editing
and become a powerful tool for biological research. Employing the CRISPR/Cas9-sgRNA …

Chromosomal rearrangements including large DNA-fragment inversions, deletions, and
duplications by Cas9 with paired sgRNAs are important to investigate genome structural …

Harnessing CRISPR-Cas9 technology provides an unprecedented ability to modify genomic
loci via DNA double-strand break (DSB) induction and repair. We analyzed nonhomologous …