Patients with suppressed immunity are at the highest risk for hospital-acquired infections.
Among these, invasive candidiasis is the most prevalent systemic fungal nosocomial …

In just three years, the green fluorescent protein (GFP) from the jellyfish Aequorea victoria
has vaulted from obscurity to become one of the most widely studied and exploited proteins …

The expression of functional proteins in heterologous hosts is a cornerstone of modern
biotechnology. Unfortunately, proteins are often difficult to express outside their original …

Tagging of genes by chromosomal integration of PCR amplified cassettes is a widely used
and fast method to label proteins in vivo in the yeast Saccharomyces cerevisiae. This …

ASH1 mRNA localizes to the bud tip in Saccharomyces cerevisiae to establish asymmetry of
HO expression, important for mating type switching. To visualize real time localization of the …

In the last 10 years green fluorescent protein (GFP) has changed from a nearly unknown
protein to a commonly used tool in molecular biology, medicine, and cell biology. GFP is …

Oral microbiota contribute to health and disease, and their disruption may influence the
course of oral diseases. Here, we used pyrosequencing to characterize the oral bacteriome …

The genome of the yeast Saccharomyces cerevisiae was the first eukaryotic genome that
has been completely sequenced (Goffeau et al., 1996). This fact, together with its easy …

We have investigated properties relevant to quantitative imaging in living cells of five green
fluorescent protein (GFP) variants that have been used extensively or are potentially useful …

Eukaryotic transcription factors (TFs) perform complex and combinatorial functions within
transcriptional networks. Here, we present a synthetic framework for systematically …