Ultrahigh-Throughput Enzyme Engineering and Discovery in In Vitro Compartments
Novel and improved biocatalysts are increasingly sourced from libraries via experimental
screening. The success of such campaigns is crucially dependent on the number of …
screening. The success of such campaigns is crucially dependent on the number of …
Cell-free enzyme cascades—application and transition from development to industrial implementation
M Teshima, VP Willers, V Sieber - Current Opinion in Biotechnology, 2023 - Elsevier
Highlights•State-of-the-art in vitro enzyme cascades to assimilate CO 2-derived C1
compounds.•Current transitioning state of the biomass-based production of commodity …
compounds.•Current transitioning state of the biomass-based production of commodity …
Ultra-high-throughput absorbance-activated droplet sorting for enzyme screening at kilohertz frequencies
EJ Medcalf, M Gantz, TS Kaminski… - Analytical …, 2023 - ACS Publications
Droplet microfluidics is a valuable method to “beat the odds” in high throughput screening
campaigns such as directed evolution, where valuable hits are infrequent and large library …
campaigns such as directed evolution, where valuable hits are infrequent and large library …
Shifting redox reaction equilibria on demand using an orthogonal redox cofactor
Nature's two redox cofactors, nicotinamide adenine dinucleotide (NAD+) and nicotinamide
adenine dinucleotide phosphate (NADP+), are held at different reduction potentials, driving …
adenine dinucleotide phosphate (NADP+), are held at different reduction potentials, driving …
Coenzyme engineering of glucose-6-phosphate dehydrogenase on a nicotinamide-based biomimic and its application as a glucose biosensor
Nicotinamide adenine dinucleotide (NAD (P)+)-dependent oxidoreductases have been
widely employed as biocatalysts for numerous applications, such as in vitro …
widely employed as biocatalysts for numerous applications, such as in vitro …
Design, construction, and application of noncanonical redox cofactor infrastructures
WB Black, S Perea, H Li - Current Opinion in Biotechnology, 2023 - Elsevier
Highlights•Noncanonical redox cofactors (NRCs) can overcome the limitations of natural
cofactors.•NRCs enable specific delivery of reducing power outside of biological …
cofactors.•NRCs enable specific delivery of reducing power outside of biological …
微液滴高通量筛选方法的研究与应用进展
秦伟彤， 杨广宇 - 合成生物学, 2023 - synbioj.cip.com.cn
在单细胞层面对生物功能进行高通量的分析和分选是对关键基因, 元件, 途径与细胞工厂进行
优化的重要技术. 基于微液滴的筛选方法因其低成本, 超高通量等优势, 已被广泛应用于生物 …
优化的重要技术. 基于微液滴的筛选方法因其低成本, 超高通量等优势, 已被广泛应用于生物 …
High-Throughput Screening Techniques for the Selection of Thermostable Enzymes
L Li, X Liu, Y Bai, B Yao, H Luo, T Tu - Journal of Agricultural and …, 2024 - ACS Publications
The acquisition of a thermostable enzyme is an indispensable prerequisite for its successful
implementation in industrial applications and the development of novel functionalities …
implementation in industrial applications and the development of novel functionalities …
[HTML][HTML] In vitro biotransformation (ivBT): definitions, opportunities, and challenges
Great needs always motivate the birth and development of new disciplines and tools. Here
we propose in vitro BioTransformation (ivBT) as a new biomanufacturing platform, between …
we propose in vitro BioTransformation (ivBT) as a new biomanufacturing platform, between …
Engineering Escherichia coli Pyruvate Metabolism to Generate Noncanonical Reducing Power
The future of biomanufacturing is dependent on rewiring biological systems to establish an
alternative approach to our current chemical industries. However, a key limitation in …
alternative approach to our current chemical industries. However, a key limitation in …