Putting precision and elegance in enzyme immobilisation with bio-orthogonal chemistry

X Pei, Z Luo, L Qiao, Q Xiao, P Zhang… - Chemical Society …, 2022 - pubs.rsc.org
The covalent immobilisation of enzymes generally involves the use of highly reactive
crosslinkers, such as glutaraldehyde, to couple enzyme molecules to each other or to …

Chemical and enzymatic methods for post-translational protein–protein conjugation

RJ Taylor, MB Geeson, T Journeaux… - Journal of the …, 2022 - ACS Publications
Fusion proteins play an essential role in the biosciences but suffer from several key
limitations, including the requirement for N-to-C terminal ligation, incompatibility of …

Spatiotemporal functional assembly of split protein pairs through a light-activated SpyLigation

ER Ruskowitz, BG Munoz-Robles, AC Strange… - Nature …, 2023 - nature.com
Proteins provide essential functional regulation of many bioprocesses across all scales of
life; however, new techniques to specifically modulate protein activity within living systems …

Hybrid small-molecule/protein fluorescent probes

M Minoshima, SI Reja, R Hashimoto, K Iijima… - Chemical …, 2024 - ACS Publications
Hybrid small-molecule/protein fluorescent probes are powerful tools for visualizing protein
localization and function in living cells. These hybrid probes are constructed by diverse site …

Irreversible light-activated SpyLigation mediates split-protein assembly in 4D

BG Munoz-Robles, CA DeForest - Nature Protocols, 2024 - nature.com
The conditional assembly of split-protein pairs to modulate biological activity is commonly
achieved by fusing split-protein fragments to dimerizing components that bring inactive pairs …

Third‐Generation Covalent TMP‐Tag for Fast Labeling and Multiplexed Imaging of Cellular Proteins

J Mo, J Chen, Y Shi, J Sun, Y Wu, T Liu… - Angewandte …, 2022 - Wiley Online Library
Self‐labeling protein tags can introduce advanced molecular motifs to specific cellular
proteins. Here we introduce the third‐generation covalent TMP‐tag (TMP‐tag3) and …

SpySwitch enables pH-or heat-responsive capture and release for plug-and-display nanoassembly

SK Vester, R Rahikainen, INA Khairil Anuar… - Nature …, 2022 - nature.com
Proteins can be empowered via SpyTag for anchoring and nanoassembly, through covalent
bonding to SpyCatcher partners. Here we generate a switchable version of SpyCatcher …

Microbial synthesis of high-molecular-weight, highly repetitive protein polymers

J Jeon, SV Subramani, KZ Lee, B Jiang… - International journal of …, 2023 - mdpi.com
High molecular weight (MW), highly repetitive protein polymers are attractive candidates to
replace petroleum-derived materials as these protein-based materials (PBMs) are …

Virus-like particles against infectious disease and cancer: guidance for the nano-architect

RA Hills, M Howarth - Current Opinion in Biotechnology, 2022 - Elsevier
Highlights•Studying VLPs gives important insights into how the immune system makes
decisions.•Spacing antigens using DNA indicates the optimal separation for B cell …

Modular capsid decoration boosts adenovirus vaccine-induced humoral immunity against SARS-CoV-2

MDJ Dicks, LM Rose, RA Russell, LAH Bowman… - Molecular Therapy, 2022 - cell.com
Adenovirus vector vaccines have been widely and successfully deployed in response to
coronavirus disease 2019 (COVID-19). However, despite inducing potent T cell immunity …