Understanding the rules of life is one of the most important scientific endeavours and has
revolutionised both biology and biotechnology. Remarkable advances in observation …

When a molecule absorbs a photon of appropriate energy, a chain of photophysical events
ensues, such as internal conversion or vibrational relaxation (loss of energy in the absence …

Conductive hydrogels are a promising class of materials to design bioelectronics for new
technological interfaces with human body, which are required to work for a long‐term or …

In vivo medical imaging has made great progress due to advances in the engineering of
imaging devices and developments in the chemistry of imaging probes. Several modalities …

Fluorescence lifetime imaging (FLIM) is a key fluorescence microscopy technique to map the
environment and interaction of fluorescent probes. It can report on photophysical events that …

Membraneless organelles are cellular compartments made from drops of liquid protein
inside a cell. These compartments assemble via the phase separation of disordered regions …

Fluorescence lifetime imaging microscopy (FLIM) and fluorescence anisotropy imaging
microscopy (FAIM) are versatile tools for the investigation of the molecular environment of …

The fusion of different protein domains via peptide linkers is a powerful, modular approach
to obtain proteins with new functions. A detailed understanding of the conformational …

Enhanced cyan fluorescent protein (ECFP) and its variant Cerulean are genetically encoded
fluorophores widely used as donors in FRET-based cell imaging experiments. First, we have …

We show that fluorescence lifetime imaging microscopy (FLIM) of green fluorescent protein
(GFP) molecules in cells can be used to report on the local refractive index of intracellular …