Single-molecule FRET methods to study the dynamics of proteins at work
Feynman commented that “Everything that living things do can be understood in terms of the
jiggling and wiggling of atoms”. Proteins can jiggle and wiggle large structural elements …
jiggling and wiggling of atoms”. Proteins can jiggle and wiggle large structural elements …
FRET-based dynamic structural biology: Challenges, perspectives and an appeal for open-science practices
Single-molecule FRET (smFRET) has become a mainstream technique for studying
biomolecular structural dynamics. The rapid and wide adoption of smFRET experiments by …
biomolecular structural dynamics. The rapid and wide adoption of smFRET experiments by …
A blind benchmark of analysis tools to infer kinetic rate constants from single-molecule FRET trajectories
Single-molecule FRET (smFRET) is a versatile technique to study the dynamics and function
of biomolecules since it makes nanoscale movements detectable as fluorescence signals …
of biomolecules since it makes nanoscale movements detectable as fluorescence signals …
Adsorption and unfolding of a single protein triggers nanoparticle aggregation
The response of living systems to nanoparticles is thought to depend on the protein corona,
which forms shortly after exposure to physiological fluids and which is linked to a wide array …
which forms shortly after exposure to physiological fluids and which is linked to a wide array …
Single-molecule imaging reveals dynamic biphasic partition of RNA-binding proteins in stress granules
B Niewidok, M Igaev, A Pereira da Graca… - Journal of Cell …, 2018 - rupress.org
Stress granules (SGs) are cytosolic, nonmembranous RNA–protein complexes. In vitro
experiments suggested that they are formed by liquid–liquid phase separation; however …
experiments suggested that they are formed by liquid–liquid phase separation; however …
Super-resolution optical fluctuation bio-imaging with dual-color carbon nanodots
AM Chizhik, S Stein, MO Dekaliuk, C Battle, W Li… - Nano …, 2016 - ACS Publications
Success in super-resolution imaging relies on a proper choice of fluorescent probes. Here,
we suggest novel easily produced and biocompatible nanoparticles carbon nanodots for …
we suggest novel easily produced and biocompatible nanoparticles carbon nanodots for …
Single molecule kinetics of bacteriorhodopsin by HS-AFM
Bacteriorhodopsin is a seven-helix light-driven proton-pump that was structurally and
functionally extensively studied. Despite a wealth of data, the single molecule kinetics of the …
functionally extensively studied. Despite a wealth of data, the single molecule kinetics of the …
Recent advances in single-molecule tracking and imaging techniques
Since the early 1990s, single-molecule detection in solution at room temperature has
enabled direct observation of single biomolecules at work in real time and under …
enabled direct observation of single biomolecules at work in real time and under …
Advantages and potential limitations of applying AFM kymograph analysis to pharmaceutically relevant membrane proteins in lipid bilayers
KG Schaefer, AG Roberts, GM King - Scientific Reports, 2023 - nature.com
Membrane proteins play critical roles in disease and in the disposition of many
pharmaceuticals. A prime example is P-glycoprotein (Pgp) which moves a diverse range of …
pharmaceuticals. A prime example is P-glycoprotein (Pgp) which moves a diverse range of …
Multi-functional DNA nanostructures that puncture and remodel lipid membranes into hybrid materials
Synthetically replicating key biological processes requires the ability to puncture lipid bilayer
membranes and to remodel their shape. Recently developed artificial DNA nanopores are …
membranes and to remodel their shape. Recently developed artificial DNA nanopores are …