Recent developments in live-cell imaging are challenging our stereotyped view of the fixed
cell nucleus. The emerging picture is that nuclear processes may rely on a constant flow of …

Fluorescence in situ hybridization combined with three-dimensional microscopy has shown
that chromosomes are not randomly strewn throughout the nucleus but are in fact fairly well …

The fission yeast Schizosaccharomyces pombe is widely used as a model system for studies
of the cell cycle and chromosome biology. To enhance these studies we have fused GFP to …

Herpes simplex virus (HSV) uses intranuclear compartmentalization to concentrate the viral
and cellular factors required for the progression of the viral life cycle. Processes as varied as …

The past decade has seen an increasing appreciation for nuclear compartmentalization as
an underlying determinant of interphase chromosome nuclear organization. To date …

Background Structural and dynamic studies of chromosomes tagged with green fluorescent
protein (GFP) in yeast and cultured animal cells have revealed some surprises. Although …

Fluorescent protein chromatin tagging as achieved by the lac operator/lac repressor system
is useful to trace distinct chromatin domains in living eukaryotic nuclei. To interpret the data …

Chromatin, once thought to serve only as a means to package DNA, is now recognized as a
major regulator of gene activity. As a result of the wide range of methods used to describe …

While the transcriptional machinery has been extensively dissected at the molecular level,
little is known about regulation of chromosomal organization in the three-dimensional space …

Chromosome localization in the interphase nuclei of eukaryotes depends on gene
replication and transcription. Little is known about chromosome localization in protozoan …