Single-molecule localization microscopy

M Lelek, MT Gyparaki, G Beliu, F Schueder… - Nature reviews …, 2021 - nature.com
Single-molecule localization microscopy (SMLM) describes a family of powerful imaging
techniques that dramatically improve spatial resolution over standard, diffraction-limited …

Biochemical and mechanical regulation of actin dynamics

P Lappalainen, T Kotila, A Jégou… - … Reviews Molecular Cell …, 2022 - nature.com
Polymerization of actin filaments against membranes produces force for numerous cellular
processes, such as migration, morphogenesis, endocytosis, phagocytosis and organelle …

Super-resolution microscopy for structural cell biology

S Liu, P Hoess, J Ries - Annual review of biophysics, 2022 - annualreviews.org
Super-resolution microscopy techniques, and specifically single-molecule localization
microscopy (SMLM), are approaching nanometer resolution inside cells and thus have great …

Generation of nanoscopic membrane curvature for membrane trafficking

MM Kozlov, JW Taraska - Nature reviews Molecular cell biology, 2023 - nature.com
Curved membranes are key features of intracellular organelles, and their generation
involves dynamic protein complexes. Here we describe the fundamental mechanisms such …

[HTML][HTML] Deep learning enables fast and dense single-molecule localization with high accuracy

A Speiser, LR Müller, P Hoess, U Matti, CJ Obara… - Nature …, 2021 - nature.com
Single-molecule localization microscopy (SMLM) has had remarkable success in imaging
cellular structures with nanometer resolution, but standard analysis algorithms require …

A review of super-resolution single-molecule localization microscopy cluster analysis and quantification methods

IM Khater, IR Nabi, G Hamarneh - Patterns, 2020 - cell.com
Single-molecule localization microscopy (SMLM) is a relatively new imaging modality,
winning the 2014 Nobel Prize in Chemistry, and considered as one of the key super …

The 2020 motile active matter roadmap

G Gompper, RG Winkler, T Speck… - Journal of Physics …, 2020 - iopscience.iop.org
Activity and autonomous motion are fundamental in living and engineering systems. This
has stimulated the new field of'active matter'in recent years, which focuses on the physical …

Super-resolution microscopy with single molecules in biology and beyond–essentials, current trends, and future challenges

L Möckl, WE Moerner - Journal of the American Chemical …, 2020 - ACS Publications
Single-molecule super-resolution microscopy has developed from a specialized technique
into one of the most versatile and powerful imaging methods of the nanoscale over the past …

[HTML][HTML] Nuclear pores as versatile reference standards for quantitative superresolution microscopy

JV Thevathasan, M Kahnwald, K Cieśliński, P Hoess… - Nature …, 2019 - nature.com
Quantitative fluorescence and superresolution microscopy are often limited by insufficient
data quality or artifacts. In this context, it is essential to have biologically relevant control …

[HTML][HTML] SMAP: a modular super-resolution microscopy analysis platform for SMLM data

J Ries - Nature Methods, 2020 - nature.com
Philosopher is scalable from laptops and desktops to high-performance servers, and can be
incorporated in workflow managers such as Galaxy-P9. The power of Philosopher is well …