In-cell structural biology aims at extracting structural information about proteins or nucleic
acids in their native, cellular environment. This emerging field holds great promise and is …

Nucleic acids are natural biopolymers of nucleotides that store, encode, transmit and
express genetic information, which play central roles in diverse cellular events and diseases …

Fluorogenic RNA aptamers are used to genetically encode fluorescent RNA and to construct
RNA-based metabolite sensors. Unlike naturally occurring aptamers that efficiently fold and …

Cryogenic electron microscopy (cryo-EM) is a promising method for characterizing the
structure of larger RNA structures and complexes. However, the structure of individual …

Highlights•Metabolic cybergenetics promises unprecedented real-time control of
fermentations.•Optogenetic regulators make ideal actuators for metabolic cybergenetics.• …

To understand the role of intracellular metabolites in cellular processes, it is important to
measure the dynamics and fluxes of small molecules in living cells. Although conventional …

Highlights•Palette of fluorogenic RNA-dye pairs now are available for biosensor
engineering.•RNA-based fluorescent biosensors developed for molecular imaging …

Sensors to measure the abundance and signaling of intracellular molecules are crucial for
understanding their physiological functions. Although conventional fluorescent protein …

The development of RNA aptamers/fluorophores system is highly desirable for
understanding the dynamic molecular biology of RNAs in vivo. Peppers-based imaging …

In situ amplification methods, such as hybridization chain reaction, are valuable tools for
mapping the spatial distribution and subcellular location of target analytes. However, the live …