The major facilitator superfamily (MFS) is the largest known superfamily of secondary active
transporters. MFS transporters are responsible for transporting a broad spectrum of …

A gene can be defined as essential when loss of its function compromises viability of the
individual (for example, embryonic lethality) or results in profound loss of fitness. At the …

To maximize a desired product, metabolic engineers typically express enzymes to high,
constant levels. Yet, permanent pathway activation can have undesirable consequences …

Precise control over microbial cell growth conditions could enable detection of minute
phenotypic changes, which would improve our understanding of how genotypes are shaped …

Cellular barcoding is a technique in which individual cells are labeled with unique nucleic
acid sequences, termed barcodes, so that they can be tracked through space and time …

The use of renewable plant biomass, lignocellulose, to produce biofuels and biochemicals
using microbial cell factories plays a fundamental role in the future bioeconomy. The …

Functional genomic strategies have become fundamental for annotating gene function and
regulatory networks. Here, we combined functional genomics with proteomics by quantifying …

CRISPR/Cas9 technology enables the rapid generation of loss‐of‐function mutations in a
targeted gene in mammalian cells. A single cell harboring those mutations can be used to …

Genome-wide phenotypic screens in the budding yeast Saccharomyces cerevisiae, enabled
by its knockout collection, have produced the largest, richest, and most systematic …

The nuclear envelope is a double membrane that separates the nucleus from the cytoplasm.
The inner nuclear membrane (INM) functions in essential nuclear processes including …