Highlights•Recent advances in single-cell proteomics.•Improvements in sample preparation,
separation techniques, mass spectrometry instrumentation, and data acquisition/analysis.• …

Mass spectrometry-based proteomics is a sophisticated identification tool specializing in
portraying protein dynamics at a molecular level. Proteomics provides biologists with a …

Identification and proteomic characterization of rare cell types within complex organ-derived
cell mixtures is best accomplished by label-free quantitative mass spectrometry. High …

Skeletal muscle is a major regulatory tissue of whole-body metabolism and is composed of a
diverse mixture of cell (fiber) types. Aging and several diseases differentially affect the …

Background Atopic dermatitis (AD) skin lesions are associated with oozing, bleeding, and
erythema. This suggests that AD is associated with vascular changes. Dupilumab is an …

The coevolution of liquid chromatography (LC) with mass spectrometry (MS) has shaped
contemporary proteomics. LC hyphenated to MS now enables quantification of more than …

High-throughput tissue proteomics has great potential in the advancement of precision
medicine. Here, we investigated the combined sensitivity of trap-elute microflow liquid …

With current trends in proteomics, especially regarding clinical and low input (to single cell)
samples, it is increasingly important to both maximize the throughput of the analysis and …

This Technical Note describes a dual-column liquid chromatography system coupled to
mass spectrometry (LC-MS) for high-throughput bottom-up proteomic analysis. This system …

Proteomics is the large scale study of protein structure and function from biological systems
through protein identification and quantification.“Shotgun proteomics” or “bottom-up …