Developing greener synthesis processes is an inescapable necessity to transform the
industrial landscape, mainly in the pharmaceutical sector, into a long-term, sustainable …

The biopharmaceutical market has experienced a tremendous growth in the last years.
However, this growth should be balanced considering the difficulty in bioproduct …

The market of biomolecules with therapeutic scopes, including peptides, is continuously
expanding. The interest towards this class of pharmaceuticals is stimulated by the broad …

Oligonucleotides (ONs) are acquiring clinical relevance and their demand is expected to
grow. However, the ON production capacity is currently limited by high manufacturing costs …

Therapeutic oligonucleotides (ONs) have great potential to treat many diseases due to their
ability to regulate gene expression. However, the inefficiency of standard purification …

The multicolumn counter-current solvent gradient purification (MCSGP) method has proven
effective in addressing the issue of elution profile overlap for difficult-to-separate proteins …

Preparative liquid chromatography in reversed phase conditions (RPLC) is the most
common approach adopted in the downstream processing for the purification of therapeutic …

Conjugation of biopharmaceuticals to polyethylene glycol chains, known as PEGylation, is
nowadays an efficient and widely exploited strategy to improve critical properties of the …

Crocin-I, a valuable natural compound found in saffron (Crocus sativus L.), is the most
abundant among the various crocin structures. Developing a cost-effective and scalable …

Highlights•The implementation of continuous chromatography can be challenging.•
Mathematical models can aid in the implementation of continuous chromatography.•Some …