Engineering biological nanopore approaches toward protein sequencing

X Wei, T Penkauskas, JE Reiner, C Kennard… - ACS …, 2023 - ACS Publications
Biotechnological innovations have vastly improved the capacity to perform large-scale
protein studies, while the methods we have for identifying and quantifying individual proteins …

Conventional and nanotechnology-based sensing methods for SARS coronavirus (2019-nCoV)

NP Shetti, A Mishra, SD Bukkitgar, S Basu… - ACS applied bio …, 2021 - ACS Publications
Ongoing pandemic coronavirus (COVID-19) has affected over 218 countries and infected
88,512,243 and 1,906,853 deaths reported by Jan. 8, 2021. At present, vaccines are being …

A click chemistry amplified nanopore assay for ultrasensitive quantification of HIV-1 p24 antigen in clinical samples

X Wei, X Wang, Z Zhang, Y Luo, Z Wang… - Nature …, 2022 - nature.com
Despite major advances in HIV testing, ultrasensitive detection of early infection remains
challenging, especially for the viral capsid protein p24, which is an early virological …

Multiplex quantitative detection of SARS-CoV-2 specific IgG and IgM antibodies based on DNA-assisted nanopore sensing

Z Zhang, X Wang, X Wei, SW Zheng, BJ Lenhart… - Biosensors and …, 2021 - Elsevier
Abstract The coronavirus disease (COVID-19) caused by severe acute respiratory syndrome
coronavirus 2 (SARS-CoV-2) has spread into a global pandemic. Early and accurate …

Protein sequencing, one molecule at a time

BM Floyd, EM Marcotte - Annual review of biophysics, 2022 - annualreviews.org
Despite tremendous gains over the past decade, methods for characterizing proteins have
generally lagged behind those for nucleic acids, which are characterized by extremely high …

Narrowing Signal Distribution by Adamantane Derivatization for Amino Acid Identification Using an α-Hemolysin Nanopore

X Wei, D Ma, J Ou, G Song, J Guo, JWF Robertson… - Nano Letters, 2024 - ACS Publications
The rapid progress in nanopore sensing has sparked interest in protein sequencing. Despite
recent notable advancements in amino acid recognition using nanopores, chemical …

Recent advances in single-entity electrochemistry for metal nanoparticle, nanodroplet, and bio-entity analysis

JW Kim, G Aruchamy, BK Kim - TrAC Trends in Analytical Chemistry, 2023 - Elsevier
The field of analytical/electroanalytical chemistry has witnessed great improvements over
the past period. Single-entity electrochemistry (SEE) was developed approximately 20 years …

Is the volume exclusion model practicable for nanopore protein sequencing?

MZ Huo, MY Li, YL Ying, YT Long - Analytical Chemistry, 2021 - ACS Publications
The nanopore approach holds the possibility for achieving single-molecule protein
sequencing. However, ongoing challenges still remain in the biological nanopore …

Recent advances in biological nanopores for nanopore sequencing, sensing and comparison of functional variations in MspA mutants

H Bhatti, R Jawed, I Ali, K Iqbal, Y Han, Z Lu, Q Liu - Rsc Advances, 2021 - pubs.rsc.org
Biological nanopores are revolutionizing human health by the great myriad of detection and
diagnostic skills. Their nano-confined area and ingenious shape are suitable to investigate a …

Quantitation of Circulating Mycobacterium tuberculosis Antigens by Nanopore Biosensing in Children Evaluated for Pulmonary Tuberculosis in South Africa

X Wang, X Wei, MM van der Zalm, Z Zhang… - ACS …, 2023 - ACS Publications
Nanopore sensing of proteomic biomarkers lacks accuracy due to the ultralow abundance of
targets, a wide variety of interferents in clinical samples, and the mismatch between pore …