Probing organoid metabolism using fluorescence lifetime imaging microscopy (FLIM): The next frontier of drug discovery and disease understanding

M Barroso, MG Monaghan, R Niesner… - Advanced Drug Delivery …, 2023 - Elsevier
Organoid models have been used to address important questions in developmental and
cancer biology, tissue repair, advanced modelling of disease and therapies, among other …

Thou shall not heal: Overcoming the non-healing behaviour of diabetic foot ulcers by engineering the inflammatory microenvironment

MG Monaghan, R Borah, C Thomsen… - Advanced Drug Delivery …, 2023 - Elsevier
Diabetic foot ulcers (DFUs) are a devastating complication for diabetic patients that have
debilitating effects and can ultimately lead to limb amputation. Healthy wounds progress …

Prediction of six macrophage phenotypes and their IL-10 content based on single-cell morphology using artificial intelligence

M Selig, L Poehlman, NC Lang, M Völker… - Frontiers in …, 2024 - frontiersin.org
Introduction The last decade has led to rapid developments and increased usage of
computational tools at the single-cell level. However, our knowledge remains limited in how …

Prediction of IDH mutation status of glioma based on terahertz spectral data

Z Sun, X Wu, R Tao, T Zhang, X Liu, J Wang… - … Acta Part A: Molecular …, 2023 - Elsevier
Gliomas are the most common type of primary tumor in the central nervous system in adults.
Isocitrate dehydrogenase (IDH) mutation status is an important molecular biomarker for adult …

Comparison of phasor analysis and biexponential decay curve fitting of autofluorescence lifetime imaging data for machine learning prediction of cellular phenotypes

L Hu, B Ter Hofstede, D Sharma, F Zhao… - Frontiers in …, 2023 - frontiersin.org
Introduction: Autofluorescence imaging of the coenzymes reduced nicotinamide (phosphate)
dinucleotide (NAD (P) H) and oxidized flavin adenine dinucleotide (FAD) provides a label …

[HTML][HTML] An assessment of the response of human MSCs to hydrostatic pressure in environments supportive of differential chondrogenesis

F Chariyev-Prinz, A Szojka, N Neto, R Burdis… - Journal of …, 2023 - Elsevier
Mechanical stimulation can modulate the chondrogenic differentiation of stem/progenitor
cells and potentially benefit tissue engineering (TE) of functional articular cartilage (AC) …

Applications of machine learning in time-domain fluorescence lifetime imaging: a review

D Gouzou, A Taimori, T Haloubi… - … and Applications in …, 2023 - iopscience.iop.org
Many medical imaging modalities have benefited from recent advances in Machine
Learning (ML), specifically in deep learning, such as neural networks. Computers can be …

Time‐Dependent Anabolic Response of hMSC‐Derived Cartilage Grafts to Hydrostatic Pressure

F Chariyev-Prinz, N Neto… - Journal of Tissue …, 2023 - Wiley Online Library
It is generally accepted that the application of hydrostatic pressure (HP) is beneficial for MSC
chondrogenesis. There is, however, evidence to suggest that the timing of application might …

Fluorescence lifetime imaging microscopy as an instrument for human sperm assessment

P Vishnyakova, E Nikonova, E Jumaniyazova… - Biochemical and …, 2023 - Elsevier
Mammalian spermatozoa are highly energized cells in which most of the proteins and
activated signaling cascades are involved in the metabolic pathways. Flavin adenine …

[HTML][HTML] Computational approaches in rheumatic diseases–Deciphering complex spatio-temporal cell interactions

C Hegarty, N Neto, P Cahill, A Floudas - Computational and Structural …, 2023 - Elsevier
Inflammatory arthritis, including rheumatoid (RA, and psoriatic (PsA) arthritis, are clinically
and immunologically heterogeneous diseases with no identified cure. Chronic inflammation …