Super-resolution fluorescence imaging by photoactivation or photoswitching of single
fluorophores and position determination (single-molecule localization microscopy, SMLM) …

The structure of the nervous system is extraordinarily complicated because individual
neurons are interconnected to hundreds or even thousands of other cells in networks that …

To fully understand how the human brain works, knowledge of its structure at high resolution
is needed. Presented here is a computationally intensive reconstruction of the ultrastructure …

Photoswitchable fluorescent probes are central to localization-based super-resolution
microscopy. Among these probes, fluorescent proteins are appealing because they are …

The importance of neuronal morphology in brain function has been recognized for over a
century. The broad applicability of" digital reconstructions" of neuron morphology across …

The transition of single-molecule fluorescence detection and imaging from in vitro to living
cells has greatly enriched our knowledge on the behavior of single biomolecules in their …

Light microscopy can be applied in vivo and can sample large tissue volumes, features
crucial for the study of single neurons and neural circuits. However, light microscopy per se …

The function of biological cells is intimately associated with their internal organization, in
which multiple subcellular structures have specialized roles. Such subcellular structures …

We introduce a method for correlative in-resin super-resolution fluorescence and electron
microscopy (EM) of biological structures in mammalian culture cells. Cryo-fixed resin …

Super‐resolution fluorescence microscopy allows for unprecedented in situ visualization of
biological structures, but its application to materials science has so far been comparatively …