CRISPRi: a way to integrate iPSC-derived neuronal models

SNJ Franks, R Heon-Roberts… - Biochemical Society …, 2024 - portlandpress.com
The genetic landscape of neurodegenerative diseases encompasses genes affecting
multiple cellular pathways which exert effects in an array of neuronal and glial cell-types …

CRISPR-based identification of N-terminal acetylation in synucleinopathies

EJ Bae, SJ Lee - Trends in Neurosciences, 2024 - cell.com
A recent study by Kumar et al. identified several biological pathways that regulate the levels
of endogenous alpha-synuclein (α-synuclein). They specifically highlighted the N-terminal …

CRISPR associated enzymes are mislocalized to the cytoplasm in iPSC-derived neurons resulting in KRAB-specific degradation

G Cajka, M Liu, OH Shalem - bioRxiv, 2024 - biorxiv.org
The use of CRISPR-associated enzymes in iPSC-derived neurons for precise gene targeting
and high-throughput gene perturbation screens offers great potential but presents unique …