▪ Abstract In just a few short years, the chemical ligation of unprotected peptide segments in
aqueous solution has established itself as the most practical method for the total synthesis of …

▪ Abstract Expressed protein ligation (EPL) is a protein engineering approach that allows
recombinant and synthetic polypeptides to be chemoselectively and regioselectively joined …

The ability to manipulate the chemical composition of proteins and peptides has been
central to the development of improved polypeptide-based therapeutics and has enabled …

Protein trans-splicing by the naturally split intein of the gene dnaE from Nostoc punctiforme
(Npu DnaE) was demonstrated here with non-native exteins in Escherichia coli. Npu DnaE …

Biophysical techniques such as fluorescence spectroscopy and nuclear magnetic
resonance (NMR) spectroscopy provide a window into the inner workings of proteins. These …

NMR spectroscopy is a key method for studying the structure and dynamics of (large)
multidomain proteins and complexes in solution. It plays a unique role in integrated …

▪ Abstract Inteins are genetic elements that disrupt the coding sequence of genes. However,
in contrast to introns, inteins are transcribed and translated together with their host protein …

The development of novel isotope labeling strategies for proteins has facilitated the study of
the structure and dynamics of these molecules. In addition, the recent emergence of …

This review focuses upon the application of solution NMR methods to multispan integral
membrane proteins, particularly with respect to determination of global folds by this …

Developments in the Field of Bioorthogonal Bond Forming Reactions—Past and Present
Trends | Bioconjugate Chemistry ACS ACS Publications C&EN CAS Find my institution Log …