Advances in X-ray crystallography methods to study structural dynamics of macromolecules

AA Kermani, S Aggarwal, A Ghanbarpour - … spectroscopic methods to study …, 2023 - Elsevier
X-ray crystallography has long been a key method in solving the three-dimensional structure
of proteins. Structural information is essential for unraveling the molecular function of …

In situ photo-patterning of pressure-resistant hydrogel membranes with controlled permeabilities in PEGDA microfluidic channels

J Decock, M Schlenk, JB Salmon - Lab on a chip, 2018 - pubs.rsc.org
We report the fabrication of highly permeable membranes in poly (ethylene glycol) diacrylate
(PEGDA) channels, for investigating ultra-or micro-filtration, at the microfluidic scale. More …

Microfluidic Microdialysis: Spatiotemporal Control over Solution Microenvironments<? format?> Using Integrated Hydrogel Membrane Microwindows

JS Paustian, RN Azevedo, STB Lundin, MJ Gilkey… - Physical Review X, 2013 - APS
We present a powerful and versatile technique that enables exquisite spatial and temporal
control over local solution chemistry in microfluidic devices. Using a microscope and a UV …

Microfluidic dialysis using photo-patterned hydrogel membranes in PDMS chips

HT Nguyen, M Massino, C Keita, JB Salmon - Lab on a Chip, 2020 - pubs.rsc.org
We report the fabrication of permeable membranes for microfluidic dialysis applications in
poly (dimethylsiloxane)(PDMS) channels. A maskless UV projection device was used to …

Real-time multiplexed PCR using surface enhanced Raman spectroscopy in a thermoplastic chip

SM Restaino, IM White - Lab on a Chip, 2018 - pubs.rsc.org
Surface enhanced Raman spectroscopy (SERS) has the potential to enable point-of-care
sensing across the spectrum of chemical and biological analytes. In diagnostic assays …

Spatial organization and mechanical properties of the pericellular matrix on chondrocytes

LT McLane, P Chang, A Granqvist, H Boehm… - Biophysical journal, 2013 - cell.com
A voluminous polymer coat adorns the surface of many eukaryotic cells. Although the
pericellular matrix (PCM) often extends several microns from the cell surface, its …

Cell surface access is modulated by tethered bottlebrush proteoglycans

PS Chang, LT McLane, R Fogg, J Scrimgeour… - Biophysical journal, 2016 - cell.com
The hyaluronan-rich pericellular matrix (PCM) plays physical and chemical roles in
biological processes ranging from brain plasticity, to adhesion-dependent phenomena such …

Dynamics of fractal cluster gels with embedded active colloids

ME Szakasits, W Zhang, MJ Solomon - Physical Review Letters, 2017 - APS
We find that embedded active colloids increase the ensemble-averaged mean squared
displacement of particles in otherwise passively fluctuating fractal cluster gels. The …

Cross polarization compatible dialysis chip

M Kornreich, M Heymann, S Fraden, R Beck - Lab on a Chip, 2014 - pubs.rsc.org
We visualize birefringence in microliter sample volumes using a microfluidic dialysis chip
optimized for cross polarization microscopy. The chip is composed of two overlapping …

Single-molecule imaging of proteoglycans in the pericellular matrix

J Scrimgeour, LT McLane, PS Chang, JE Curtis - Biophysical Journal, 2017 - cell.com
The pericellular matrix is a robust, hyaluronan-rich polymer brush-like structure that controls
access to the cell surface, and plays an important role in cell adhesion, migration, and …