Production of recombinant proteins in Bacillus subtilis

W Schumann - Advances in applied microbiology, 2007 - Elsevier
Publisher Summary The classical way of protein purification starting from a large number of
cells producing the protein using its authentic expression signals has been replaced by …

Mosquitocidal toxins of bacilli and their genetic manipulation for effective biological control of mosquitoes

AG Porter, EW Davidson, JW Liu - Microbiological Reviews, 1993 - Am Soc Microbiol
The identification, cloning, and characterization of protein toxins from various species of
bacilli have demonstrated the existence of mosquitocidal toxins with different structures …

The consequences of growth of a mutator strain of Escherichia coli as measured by loss of function among multiple gene targets and loss of fitness

P Funchain, A Yeung, J Lee Stewart, R Lin… - Genetics, 2000 - academic.oup.com
We have examined the composition of members of mutator populations of Escherichia coli
by employing an extensive set of phenotypic screens that allow us to monitor the function of> …

Integrational Vectors for Genetic Manipulation in Bacillus subtilis

M Perego - Bacillus subtilis and Other Gram‐Positive Bacteria …, 1993 - Wiley Online Library
The development of integrational vectors has provided a versatile system for the advance of
molecular genetic and mutagenic studies in Bacillus subtilis. Over the past decade, the …

Construction of an integration vector for use in the archaebacterium Methanococcus voltae and expression of a eubacterial resistance gene

P Gernhardt, O Possot, M Foglino, L Sibold… - Molecular and General …, 1990 - Springer
An integration vector for use in Methanococcus voltae was constructed, based on the
Escherichia coli vector pUC18. It carries the structural gene for puromycin transacetylase …

Integration and expression of alpha-amylase and endoglucanase genes in the Lactobacillus plantarum chromosome

T Scheirlinck, J Mahillon, H Joos… - Applied and …, 1989 - Am Soc Microbiol
A commercial grass silage starter strain of Lactobacillus plantarum was transformed by high-
frequency electroporation with plasmids containing an alpha-amylase gene from Bacillus …

Campbell-like integration of heterologous plasmid DNA into the chromosome of Lactococcus lactis subsp. lactis

KJ Leenhouts, J Kok, G Venema - Applied and environmental …, 1989 - Am Soc Microbiol
Integrable vectors were constructed based on the plasmid pHV60, which is essentially a
pBR322 replicon carrying a chloramphenicol resistance marker, by inserting 1.3-kilobase …

[图书][B] Bacillus

CR Harwood - 1989 - books.google.com
The genus Bacillw; has a long history of importance, both from an economic point of view
and as a source of experimental microorganisms. This volume critically reviews aspects of …

Increased production of Bacillus keratinase by chromosomal integration of multiple copies of the kerA gene

JJ Wang, K Rojanatavorn… - Biotechnology and …, 2004 - Wiley Online Library
To increase the production of keratinase, stable strains of Bacillus licheniformis carrying
multiple keratinase gene copies in the chromosome were developed. Integrative vectors …

Effect of stage 0 sporulation mutations on subtilisin expression

E Ferrari, SM Howard, JA Hoch - Journal of bacteriology, 1986 - Am Soc Microbiol
Subtilisin expression as a function of growth and sporulation was determined using a
presubtilisin-beta-galactosidase gene fusion. An approximately 500-base-pair region …