[HTML][HTML] Clinical and immunological effects of mRNA vaccines in malignant diseases
In vitro-transcribed messenger RNA-based therapeutics represent a relatively novel and
highly efficient class of drugs. Several recently published studies emphasize the potential …
highly efficient class of drugs. Several recently published studies emphasize the potential …
Emergence of synthetic mRNA: In vitro synthesis of mRNA and its applications in regenerative medicine
The field of gene therapy has evolved over the past two decades after the first introduction of
nucleic acid drugs, such as plasmid DNA (pDNA). With the development of in vitro …
nucleic acid drugs, such as plasmid DNA (pDNA). With the development of in vitro …
Bacterial retrons encode phage-defending tripartite toxin–antitoxin systems
Retrons are prokaryotic genetic retroelements encoding a reverse transcriptase that
produces multi-copy single-stranded DNA (msDNA). Despite decades of research on the …
produces multi-copy single-stranded DNA (msDNA). Despite decades of research on the …
Generating the optimal mRNA for therapy: HPLC purification eliminates immune activation and improves translation of nucleoside-modified, protein-encoding mRNA
In vitro-transcribed mRNA has great therapeutic potential to transiently express the encoded
protein without the adverse effects of viral and DNA-based constructs. Mammalian cells …
protein without the adverse effects of viral and DNA-based constructs. Mammalian cells …
Reduced stability and intracellular transport of dsRNA contribute to poor RNAi response in lepidopteran insects
ABSTRACT RNA interference (RNAi) has become a widely used reverse genetic tool to
study gene function in eukaryotic organisms and is being developed as a technology for …
study gene function in eukaryotic organisms and is being developed as a technology for …
Riboregulated toehold-gated gRNA for programmable CRISPR–Cas9 function
Predictable control over gene expression is essential to elicit desired synthetic cellular
phenotypes. Although CRISPR–Cas9 offers a simple RNA-guided method for targeted …
phenotypes. Although CRISPR–Cas9 offers a simple RNA-guided method for targeted …
Environmental fate of RNA interference pesticides: adsorption and degradation of double-stranded RNA molecules in agricultural soils
KM Parker, V Barragán Borrero… - … science & technology, 2019 - ACS Publications
Double-stranded RNA (dsRNA) pesticides are a new generation of crop protectants that
interfere with protein expression in targeted pest insects by a cellular mechanism called …
interfere with protein expression in targeted pest insects by a cellular mechanism called …
[HTML][HTML] A long non-coding RNA is required for targeting centromeric protein A to the human centromere
D Quénet, Y Dalal - elife, 2014 - elifesciences.org
The centromere is a specialized chromatin region marked by the histone H3 variant CENP-
A. Although active centromeric transcription has been documented for over a decade, the …
A. Although active centromeric transcription has been documented for over a decade, the …
Polyphosphate nanoparticles on the platelet surface trigger contact system activation
JJF Verhoef, AD Barendrecht, KF Nickel… - Blood, The Journal …, 2017 - ashpublications.org
Polyphosphate is an inorganic polymer that can potentiate several interactions in the blood
coagulation system. Blood platelets contain polyphosphate, and the secretion of platelet …
coagulation system. Blood platelets contain polyphosphate, and the secretion of platelet …
HPLC purification of in vitro transcribed long RNA
D Weissman, N Pardi, H Muramatsu… - Synthetic messenger RNA …, 2013 - Springer
In vitro transcription of DNA with phage RNA polymerases is currently the most efficient
method to produce long sequence-specific RNA. While the reaction can yield large …
method to produce long sequence-specific RNA. While the reaction can yield large …