Single-molecule localization microscopy (SMLM) describes a family of powerful imaging
techniques that dramatically improve spatial resolution over standard, diffraction-limited …

Super-resolution microscopy techniques, and specifically single-molecule localization
microscopy (SMLM), are approaching nanometer resolution inside cells and thus have great …

With the widespread uptake of two-dimensional (2D) and three-dimensional (3D) single-
molecule localization microscopy (SMLM), a large set of different data analysis packages …

Quantitative fluorescence and superresolution microscopy are often limited by insufficient
data quality or artifacts. In this context, it is essential to have biologically relevant control …

The past decade has witnessed an explosion in the use of super-resolution fluorescence
microscopy methods in biology and other fields. Single-molecule localization microscopy …

Observation of molecular processes inside living cells is fundamental to a quantitative
understanding of how biological systems function. Specifically, decoding the complex …

Counting molecules in complexes is challenging, even with super-resolution microscopy.
Here, we use the programmable and specific binding of dye-labeled DNA probes to count …

Since its initial demonstration in 2000, far-field super-resolution light microscopy has
undergone tremendous technological developments. In parallel, these developments have …

Measurement science has been converging to smaller and smaller samples, such that it is
now possible to detect single molecules. This Review focuses on the next generation of …

Super-resolution microscopy (SRM) methods have allowed scientists to exceed the
diffraction limit of light, enabling the discovery and investigation of cellular structures at the …