Super-resolution microscopy demystified

L Schermelleh, A Ferrand, T Huser, C Eggeling… - Nature cell …, 2019 - nature.com
Super-resolution microscopy (SRM) bypasses the diffraction limit, a physical barrier that
restricts the optical resolution to roughly 250 nm and was previously thought to be …

The cell biologist's guide to super-resolution microscopy

G Jacquemet, AF Carisey, H Hamidi… - Journal of Cell …, 2020 - journals.biologists.com
Fluorescence microscopy has become a ubiquitous method to observe the location of
specific molecular components within cells. However, the resolution of light microscopy is …

Fluorescence lifetime imaging for studying DNA compaction and gene activities

SM Levchenko, A Pliss, X Peng, PN Prasad… - Light: Science & …, 2021 - nature.com
Optical imaging is a most useful and widespread technique for the investigation of the
structure and function of the cellular genomes. However, an analysis of immensely …

Calibrating evanescent-wave penetration depths for biological TIRF microscopy

M Oheim, A Salomon, A Weissman, M Brunstein… - Biophysical …, 2019 - cell.com
Roughly half of a cell's proteins are located at or near the plasma membrane. In this
restricted space, the cell senses its environment, signals to its neighbors, and exchanges …

[PDF][PDF] 三维超分辨显微成像技术的研究进展及展望

王潇, 涂世杰, 刘鑫, 赵悦晗, 匡翠方… - Laser & …, 2021 - researching.cn
摘要超分辨显微成像技术是细胞生物学中研究细胞器结构, 相互作用和蛋白质功能的强大工具,
其具有突破光学衍射极限的分辨能力, 从纳米尺度上为细胞生物学提供了新的分析手段 …

Fast-tracking of single emitters in large volumes with nanometer precision

B Louis, R Camacho, R Bresolí-Obach, S Abakumov… - Optics …, 2020 - opg.optica.org
Multifocal plane microscopy allows for capturing images at different focal planes
simultaneously. Using a proprietary prism which splits the emitted light into paths of different …

Three-dimensional, dual-color nanoscopy enabled by migrating photon avalanches with one single low-power CW beam

Z Zhu, Y Liang, Q Zhao, H Wu, B Pan, S Qiao, B Wang… - Science Bulletin, 2024 - Elsevier
The development of super-resolution fluorescence microscopy is very essential for
understanding the physical and biological fundamentals at nanometer scale. However, to …

Cortical actin dynamics in endothelial permeability

P Belvitch, YM Htwe, ME Brown, S Dudek - Current topics in membranes, 2018 - Elsevier
The pulmonary endothelial cell forms a critical semi-permeable barrier between the vascular
and interstitial space. As part of the blood-gas barrier in the lung, the endothelium plays a …

Observing single cells in whole organs with optical imaging

X Yang, T Jiang, L Liu, X Zhao, X Yu… - Journal of Innovative …, 2023 - World Scientific
Cells are the basic unit of human organs that are not fully understood. The revolutionary
advancements of optical imaging allowed us to observe single cells in whole organs …

Theoretical and practical guide for an axial superresolved focus via Gouy phase steering

X Liu, X Xia, Z Yao, T Zhang, M Jiang, Q Yang… - Photonics …, 2022 - opg.optica.org
Achieving an axial superresolved focus with a single lens by simply inserting a modulation
mask in the pupil plane is preferred due to its compact configuration and general …