A robust platform for facile defined glycan synthesis does not exist. Yet the need for such
technology has never been greater as researchers seek to understand the full scope of …

Aims This review is intended to update our previous discussion on the chemoenzymatic
synthesis of carbohydrates and glycoconjugates,(1) with new emphasis placed on …

Glycosylation can significantly improve the physicochemical and biological properties of
small molecules like vitamins, antibiotics, flavors, and fragrances. The chemical synthesis of …

Enzymes are nature's catalyst of choice for the highly selective and efficient coupling of
carbohydrates. Enzymatic sugar coupling is a competitive technology for industrial …

In recent years, substantial advances have been made in the engineering of glycosidases
and glycosyltransferases for the synthesis and degradation of glycan structures. Key …

Oligosaccharide synthesis is becoming increasingly important to the pharmaceutical
industry because these biomolecules have potential application as therapeutics. Enzymatic …

The Agrobacterium sp. β-glucosidase (Abg) is a retaining β-glycosidase and its nucleophile
mutants, termed Abg glycosynthases, catalyze the formation of glycosidic bonds using α …

Fucosyloligosaccharides have great therapeutic potential. Here we present a new route for
synthesizing a Fucα1, 2Gal linkage by introducing glycosynthase technology into 1, 2-α-l …

The inhibition kinetics for isoacarbose (a pseudotetrasaccharide, IsoAca) and acarviosine–
glucose (pseudotrisaccharide, AcvGlc), both of which are derivatives of acarbose, were …

The α-l-fucosidase from Thermotoga maritima (Tmαfuc) was converted into α-l-
transfucosidase variants by directed evolution. The wild-type enzyme catalyzes …