The many faces of RNA-based RNase P, an RNA-world relic
HD Phan, LB Lai, WJ Zahurancik, V Gopalan - Trends in biochemical …, 2021 - cell.com
RNase P is an essential enzyme that catalyzes removal of the 5′ leader from precursor
transfer RNAs. The ribonucleoprotein (RNP) form of RNase P is present in all domains of life …
transfer RNAs. The ribonucleoprotein (RNP) form of RNase P is present in all domains of life …
[HTML][HTML] The discovery of a catalytic RNA within RNase P and its legacy
Sidney Altman's discovery of the processing of one RNA by another RNA that acts like an
enzyme was revolutionary in biology and the basis for his sharing the 1989 Nobel Prize in …
enzyme was revolutionary in biology and the basis for his sharing the 1989 Nobel Prize in …
[HTML][HTML] Importance of residue 248 in Escherichia coli RNase P RNA mediated cleavage
Abstract tRNA genes are transcribed as precursors and RNase P generates the matured
5'end of tRNAs. It has been suggested that residue− 1 (the residue immediately 5ʹ of the …
5'end of tRNAs. It has been suggested that residue− 1 (the residue immediately 5ʹ of the …
[HTML][HTML] Cryo-electron microscopy structure of an archaeal ribonuclease P holoenzyme
F Wan, Q Wang, J Tan, M Tan, J Chen, S Shi… - Nature …, 2019 - nature.com
Abstract Ribonuclease P (RNase P) is an essential ribozyme responsible for tRNA 5′
maturation. Here we report the cryo-EM structures of Methanocaldococcus jannaschii (Mja) …
maturation. Here we report the cryo-EM structures of Methanocaldococcus jannaschii (Mja) …
Characterization of RNA-based and protein-only RNases P from bacteria encoding both enzyme types
M Gößringer, NB Wäber, JC Wiegard, RK Hartmann - RNA, 2023 - rnajournal.cshlp.org
A small group of bacteria encode two types of RNase P, the classical ribonucleoprotein
(RNP) RNase P as well as the protein-only RNase P HARP (h omolog of A quifex R Nase P) …
(RNP) RNase P as well as the protein-only RNase P HARP (h omolog of A quifex R Nase P) …
Divergent molecular assembly and catalytic mechanisms between bacterial and archaeal RNase P in pre-tRNA cleavage
X Liang, D Chen, A Su, Y Liu - Proceedings of the National Academy of …, 2024 - pnas.org
Ribonuclease P (RNase P) plays a vital role in the maturation of tRNA across bacteria,
archaea, and eukaryotes. However, how RNase P assembles various components to …
archaea, and eukaryotes. However, how RNase P assembles various components to …
Protein cofactors and substrate influence Mg2+-dependent structural changes in the catalytic RNA of archaeal RNase P
IA Marathe, SM Lai, WJ Zahurancik… - Nucleic acids …, 2021 - academic.oup.com
The ribonucleoprotein (RNP) form of archaeal RNase P comprises one catalytic RNA and
five protein cofactors. To catalyze Mg2+-dependent cleavage of the 5′ leader from pre …
five protein cofactors. To catalyze Mg2+-dependent cleavage of the 5′ leader from pre …
Multiple structural flavors of RNase P in precursor tRNA processing
S Sridhara - Wiley Interdisciplinary Reviews: RNA, 2024 - Wiley Online Library
The precursor transfer RNAs (pre‐tRNAs) require extensive processing to generate mature
tRNAs possessing proper fold, structural stability, and functionality required to sustain …
tRNAs possessing proper fold, structural stability, and functionality required to sustain …
[HTML][HTML] A minimal RNA substrate with dual fluorescent probes enables rapid kinetics and provides insight into bacterial RNase P active site interactions
Bacterial ribonuclease P (RNase P) is a tRNA processing endonuclease that occurs
primarily as a ribonucleoprotein with a catalytic RNA subunit (P RNA). As one of the first …
primarily as a ribonucleoprotein with a catalytic RNA subunit (P RNA). As one of the first …
Comparative study on tertiary contacts and folding of RNase P RNAs from a psychrophilic, a mesophilic/radiation-resistant, and a thermophilic bacterium
M Marszalkowski, A Werner, R Feltens, D Helmecke… - RNA, 2021 - rnajournal.cshlp.org
In most bacterial type A RNase P RNAs (P RNAs), two major loop-helix tertiary contacts (L8–
P4 and L18–P8) help to orient the two independently folding S-and C-domains for concerted …
P4 and L18–P8) help to orient the two independently folding S-and C-domains for concerted …