Technical pitfalls when collecting, cryopreserving, thawing, and stimulating human T-cells

DJ Browne, CM Miller, DL Doolan - Frontiers in Immunology, 2024 - frontiersin.org
The collection, cryopreservation, thawing, and culture of peripheral blood mononuclear cells
(PBMCs) can profoundly influence T cell viability and immunogenicity. Gold-standard PBMC …

Natural antibodies produced in vaccinated patients and COVID-19 convalescents recognize and hydrolyze oligopeptides corresponding to the S-protein of SARS-CoV …

AM Timofeeva, SE Sedykh, TA Sedykh, GA Nevinsky - Vaccines, 2023 - mdpi.com
The S-protein is the major antigen of the SARS-CoV-2 virus, against which protective
antibodies are generated. The S-protein gene was used in adenoviral vectors and mRNA …

BBIBP-corV vaccination against the SARS-coV-2 virus affects the gut microbiome

Y Shen, Y Dong, J Jiao, P Wang, M Chen, J Li - Vaccines, 2023 - mdpi.com
Several observational studies have confirmed that the severe acute respiratory syndrome
coronavirus2 (SARS-CoV-2) might substantially affect the gastrointestinal (GI) system by …

Identification of Antibody-Mediated Hydrolysis Sites of Oligopeptides Corresponding to the SARS-CoV-2 S-Protein by MALDI-TOF Mass Spectrometry

AM Timofeeva, SE Sedykh, PS Dmitrenok… - International Journal of …, 2023 - mdpi.com
Antibodies recognizing RBD and the S-protein have been previously demonstrated to be
formed in humans after SARS-CoV-2 infection and vaccination with the Sputnik V …

Reverse vaccinology and immunoinformatic approach for designing a bivalent vaccine candidate against hepatitis A and hepatitis B viruses

N Ahmadi, M Aghasadeghi, M Hamidi-Fard… - Molecular …, 2023 - Springer
Hepatitis A and B are two crucial viral infections that still dramatically affect public health
worldwide. Hepatitis A Virus (HAV) is the main cause of acute hepatitis, whereas Hepatitis B …

Peptide-ligand conjugate based immunotherapeutic approach for targeted dismissal of non-structural protein 1 of dengue virus: A novel therapeutic solution for mild …

V Srivastava, P Godara, SP Jena, B Naik… - International Journal of …, 2024 - Elsevier
Dengue virus infection has significantly increased, with reported cases soaring from 505,430
in 2000 to 2,809,818 in 2022, emphasizing the need for effective treatments. Among the …

Lessons from COVID‐19: Aspects of prevention, therapeutics, and diagnostics against SARS‐CoV‐2 with special focus on JN. 1 and XBB sublineages

P Jaishwal, U Gyaneshwari, K Jha… - MedComm–Future …, 2024 - Wiley Online Library
The end of second decade in the 21st century witnessed a prominent disease outbreak
caused by the novel coronavirus SARS‐CoV‐2 (including most diverse omicron …