Examining host–pathogen interactions in animals can capture aspects of infection that are
obscured in cell culture. Using CRISPR-based screens, we functionally profile the entire …

DNA-amplicon-based microbiota profiling can estimate species diversity and abundance but
cannot resolve genetic differences within individuals of the same species. Here we report …

Within a host, pathogens encounter a diverse and changing landscape of cell types,
nutrients, and immune responses. Examining host-pathogen interactions in animal models …

Listeria monocytogenes is a bacterial pathogen that can cause life-threatening central
nervous system (CNS) infections. While mechanisms by which L. monocytogenes and other …

Using a CRISPR/Cas9-based method, Wincott et al. generated a stable, complex
Toxoplasma gondii population composed of 96 barcoded clonal lineages. By tracking the …

Bacteremia, a leading cause of death, generally arises after bacteria establish infection in a
particular tissue and transit to secondary sites. Studying dissemination from primary sites by …

Aneuploidy is generally considered harmful, but in some microorganisms, it can act as an
adaptive mechanism against environmental stresses. Here, we used Leishmania–a …

Cellular Barcoding of Protozoan Pathogens for Within-Host Population Dynamics and in vivo
Drug Discovery Page 1 Imperial College London Doctoral Thesis Cellular Barcoding of …

Protozoan pathogens such as Plasmodium spp., Leishmania spp., Toxoplasma gondii, and
Trypanosoma spp. are often associated with high-mortality, acute and chronic diseases of …