Electrostatic interactions in protein structure, folding, binding, and condensation

HX Zhou, X Pang - Chemical reviews, 2018 - ACS Publications
Charged and polar groups, through forming ion pairs, hydrogen bonds, and other less
specific electrostatic interactions, impart important properties to proteins. Modulation of the …

In-cell structural biology by NMR: the benefits of the atomic scale

FX Theillet - Chemical reviews, 2022 - ACS Publications
In-cell structural biology aims at extracting structural information about proteins or nucleic
acids in their native, cellular environment. This emerging field holds great promise and is …

[HTML][HTML] Transcription factors activate genes through the phase-separation capacity of their activation domains

A Boija, IA Klein, BR Sabari, A Dall'Agnese, EL Coffey… - Cell, 2018 - cell.com
Gene expression is controlled by transcription factors (TFs) that consist of DNA-binding
domains (DBDs) and activation domains (ADs). The DBDs have been well characterized …

Phase separation drives RNA virus-induced activation of the NLRP6 inflammasome

C Shen, R Li, R Negro, J Cheng, SM Vora, TM Fu… - Cell, 2021 - cell.com
NLRP6 is important in host defense by inducing functional outcomes including
inflammasome activation and interferon production. Here, we show that NLRP6 undergoes …

[HTML][HTML] De novo engineering of intracellular condensates using artificial disordered proteins

M Dzuricky, BA Rogers, A Shahid, PS Cremer… - Nature …, 2020 - nature.com
Phase separation of intrinsically disordered proteins (IDPs) is a remarkable feature of living
cells to dynamically control intracellular partitioning. Despite the numerous new IDPs that …

Intrinsically disordered proteins: critical components of the wetware

P Kulkarni, S Bhattacharya, S Achuthan… - Chemical …, 2022 - ACS Publications
Despite the wealth of knowledge gained about intrinsically disordered proteins (IDPs) since
their discovery, there are several aspects that remain unexplored and, hence, poorly …

Physics-based computational and theoretical approaches to intrinsically disordered proteins

JE Shea, RB Best, J Mittal - Current opinion in structural biology, 2021 - Elsevier
Highlights•Liquid-liquid phase separation underlies the formation of membraneless
organelles within cells.•Theory and molecular simulations help to relate interactions at the …

Microglial-derived C1q integrates into neuronal ribonucleoprotein complexes and impacts protein homeostasis in the aging brain

N Scott-Hewitt, M Mahoney, Y Huang, N Korte… - Cell, 2024 - cell.com
Neuroimmune interactions mediate intercellular communication and underlie critical brain
functions. Microglia, CNS-resident macrophages, modulate the brain through direct physical …

DisProt: intrinsic protein disorder annotation in 2020

A Hatos, B Hajdu-Soltész, AM Monzon… - Nucleic acids …, 2020 - academic.oup.com
Abstract The Database of Protein Disorder (DisProt, URL: https://disprot. org) provides
manually curated annotations of intrinsically disordered proteins from the literature. Here we …

Interaction dynamics of intrinsically disordered proteins from single-molecule spectroscopy

A Chowdhury, D Nettels… - Annual review of …, 2023 - annualreviews.org
Many proteins contain large structurally disordered regions or are entirely disordered under
physiological conditions. The functions of these intrinsically disordered proteins (IDPs) often …